| 浊点萃取-高效液相色谱法同时测定药片与尿样中异烟肼、利福平和吡嗪酰胺 |
| |
| 引用本文： | 杭菊英,周智明,赵道远,杨明敏.浊点萃取-高效液相色谱法同时测定药片与尿样中异烟肼、利福平和吡嗪酰胺[J].分析化学,2009,37(6). |
| |
| 作者姓名： | 杭菊英 周智明 赵道远 杨明敏 |
| |
| 作者单位： | 南京农业大学理学院,南京,210095 |
| |
| 摘 要： | 建立了以聚乙二醇(PEG)6000为萃取剂的浊点萃取(CPE)-反相高效液相色谱同时测定异烟肼、利福平和吡嗪酰胺的方法,由于异烟肼不易被浊点萃取方法富集,使异烟肼与对氯苯甲醛在酸性条件下反应衍生为异烟肼腙,异烟肼腙在液相色谱上的信号与异烟肼浓度存在着线性关系.在优化的CPE条件下,采用梯度洗脱的方式,用紫外检测器在310 nm波长下,异烟肼、吡嗪酰胺和利福平的保留时间分别为14.5、34.4和38.1 min,定量范围分别是0.01～10 mg/L、0.08～25 mg/L和0.05～20 mg/L.线性相关系数r>0.99.3种药的平均回收率范围为91.50%～98.75%,精密度(RSD)为 0.22%～1.54%.异烟肼、利福平和吡嗪酰胺的检出限分别为4×10-3、2×10-2和3×10-2 mg/L.本法用于药片和尿样中3种药物的检测,结果满意.
|
| 关 键 词： | 浊点萃取 液相色谱 异烟肼 利福平 吡嗪酰胺 |
Simultaneous Determination of Isoniazid,Rifampicin and Pyrazinamide in Pharmaceuticals and Human Urine by Cloud Point Extraction High Performance Liquid Chromatography |
| |
| HANG Ju-Ying,ZHOU Zhi-Ming,ZHAO Dao-Yuan,YANG Ming-Min.Simultaneous Determination of Isoniazid,Rifampicin and Pyrazinamide in Pharmaceuticals and Human Urine by Cloud Point Extraction High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2009,37(6). |
| |
| Authors: | HANG Ju-Ying ZHOU Zhi-Ming ZHAO Dao-Yuan YANG Ming-Min |
| |
| Institution: | College of Science;Nanjing Agricultural University;Nanjing 210095 |
| |
| Abstract: | Isoniazid(IN) can not be sufficiently extracted by the cloud point extraction(CPE) of polylethyleneglycol(PEG) 6000,and reacts with para-chlorobenzaldehyde to form a derivative(para-chlorobenzaldehyde-isonicotiny-hydrazone,CIH).It was proven that there was linear relationship between the signal of CIH and the concentration of IN.A cloud point extraction system of polylethyleneglycol 6000(NH4)2SO4 coupled with high performance liquid chromatography was developed for the separation and the simultaneous determ... |
| |
| Keywords: | Cloud point extraction high performance liquid chromatography isoniazid rifampicin pyrazinamide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
