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| 醚型菊酯类农药通用抗原的合成及鉴定 | |
| 引用本文： | 睢珂,潘家荣,王磊,王志伟,赵杰,郑月明,诸葛洁婧,潘文斌.醚型菊酯类农药通用抗原的合成及鉴定[J].分析化学,2012,40(8):1274-1278. |
| 作者姓名： | 睢珂 潘家荣 王磊 王志伟 赵杰 郑月明 诸葛洁婧 潘文斌 |
| 作者单位： | 1. 中国农业科学院农产品加工研究所,北京,100193 2. 中国农业科学院农产品加工研究所,北京100193;中国计量学院生命科学学院,杭州310018 3. 中国农业科学院农产品加工研究所,北京100193;中国计量学院生命科学学院,杭州310018;沈阳农业大学研究生部,沈阳110866 4. 中国计量学院生命科学学院,杭州,310018 |
| 基金项目： | “十一五”国家科技支撑计划项目 |
| 摘 要： | 以2-(4-乙氧基苯基)-2-甲基丙醇和氯乙酸钠为原料,合成了醚型菊酯类农药通用半抗原Hapten I,经1 H-NMR及13C-NMR鉴定后,分别与牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联,制得免疫原和包被原,经紫外光谱分析法计算得其偶联比分别为14∶1和35∶1,说明人工抗原合成成功.免疫Balb/c小鼠制备多克隆抗体,效价达1.28×105,用半抗原经间接竞争ELISA检测人工抗原的免疫原性,IC50和IC10值分别为0.2653和0.0012 mg/L,证明人工抗原具有较好的免疫原性.交叉反应表明此多克隆抗体具有良好的特异性. |
| 关 键 词： | 醚型菊酯类农药 通用抗原 酶联免疫吸附法 |
Synthesis and Identification of Generic Antigen for Ether Pyrethroid Pesticides |
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| SUI Ke , PAN Jia-Rong , WANG Lei , WANG Zhi-Wei , ZHAO Jie , ZHEN Yue-Ming , ZHUGE Jie-Jing , PAN Wen-Bin.Synthesis and Identification of Generic Antigen for Ether Pyrethroid Pesticides[J].Chinese Journal of Analytical Chemistry,2012,40(8):1274-1278. | |
| Authors: | SUI Ke PAN Jia-Rong WANG Lei WANG Zhi-Wei ZHAO Jie ZHEN Yue-Ming ZHUGE Jie-Jing PAN Wen-Bin |
| Institution: | 1(Agro-food processing Institute,Chinese Academy of Agricultural Sciences,Beijing 100193,China) 2(College of life sciences,China Jiliang University,Hangzhou 310018,China) 3(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China) |
| Abstract: | 2-(4-Ethoxyphenyl)-2-methylpropanol can react with sodium chloroacetate by nuclephilic substitution reaction.HaptenⅠ,as the product of the above reaction identified by 1H-NMR and 13C-NMR,was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) by the active ester method to get artificial immune antigen and coating antigen,respectively.The binding ratio of artificial antigens was detected by UV spectroscopy as 14 ∶ 1 and 35 ∶ 1.Balb/c mice were immunized with immunogen to prepare antiserum whose titer reached 1.28×105.The icELISA was conducted with the antiserum,and the IC50 was 0.2653 mg/L and the IC10 was 0.0012 mg/L. |
| Keywords: | Ether pyrethroid pesticides Generic antigen Enzyme-linked immunosorbent assay |
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