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| 微电极阵列芯片的脂质体电融合研究 | |
| 引用本文： | 蒋凤,杨军,王振宇,胡宁,郑小林,谢琳,杨忠,陈洁.微电极阵列芯片的脂质体电融合研究[J].分析化学,2012,40(4):551-555. |
| 作者姓名： | 蒋凤 杨军 王振宇 胡宁 郑小林 谢琳 杨忠 陈洁 |
| 作者单位： | 1. 重庆大学生物工程学院,生物流变科学与技术教育部重点实验室,重庆400030 2. 第三军医大学大坪医院野战外科研究所眼科,重庆,400042 3. 第三军医大学基础医学部组织学与胚胎学教研室,重庆,400038 4. 重庆医科大学附属儿童医院儿童营养研究中心,重庆,400014 |
| 基金项目： | 国家自然科学基金(No.81071278);教育部“新世纪优秀人才支持计划”(No.NCET-09-0842);重庆市科技计划(No.CSTC2009AB5081);重庆大学研究生创新团队建设(No.200909A1002)资助项目 |
| 摘 要： | 利用旋转蒸发法制作基于大豆卵磷脂的一种大型脂质体,在微电极阵列芯片上进行脂质体电融合实验研究.在电融合过程中,利用介电电泳力实现脂质体在微流控芯片中的排队,再利用高场强的电脉冲使脂质体膜发生可逆性电穿孔,在持续的介电电泳力作用下,使穿孔的脂质体实现融合.芯片上脂质体的融合率可以达到20％左右.而且,玻璃基底材料和低深宽比的通道结构更有利于脂质体融合过程的观察与控制. |
| 关 键 词： | 微流控 脂质体 介电电泳 电融合 微电极 |
Study of Liposome Electrofusion on Microelectrode Array Chip |
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| JIANG Feng , YANG Jun , WANG Zhen-Yu , HU Ning , ZHENG Xiao-Lin , XIE Lin , YANG Zhong , CHEN Jie.Study of Liposome Electrofusion on Microelectrode Array Chip[J].Chinese Journal of Analytical Chemistry,2012,40(4):551-555. | |
| Authors: | JIANG Feng YANG Jun WANG Zhen-Yu HU Ning ZHENG Xiao-Lin XIE Lin YANG Zhong CHEN Jie |
| Institution: | 1(Key Laboratory of Biorheological Science and Technology,Ministry of Education, Bioengineering College,Chongqing University,Chongqing 400030,China) 2(Department of Ophthalmology,Research Institute of Field and Surgery of Daping Hospital, Third Military Medical University,Chongqing 400042,China) 3(Department of Histology and Embryology,Third Military Medical University,Chongqing 400038,China) 4(Nutritional Research Center,Children′s Hospital of Chongqing Medical University,Chongqing 400014,China) |
| Abstract: | Large liposomes that electrofused on a microelectrode array based microchip were prepared by using soybean lecithin and the co-evaporating method.In the electrofusion process,liposomes were aligned by the dielectrophoretic force,electroporated by using high-strength electric pulses,and subsequently fused by using continuous dielectrophoretic force.Experimental results showed that 20% liposomes could be fused on the chip,and the glass substrate and low depth-width ratio channel were helpful for the observation and control of the liposome fusion. |
| Keywords: | Microfluidics Liposome Dielectrophoresis Electrofusion Microelectrode |
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