| 微流控二维电泳芯片的电压控制优化 |
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| 引用本文： | 梁恒,范军,李涓,沈燕.微流控二维电泳芯片的电压控制优化[J].分析化学,2009,37(10). |
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| 作者姓名： | 梁恒 范军 李涓 沈燕 |
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| 作者单位： | 西安交通大学生物医学信息工程教育部重点实验室,分离科学研究所,西安,710049 |
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| 基金项目： | 国家自然科学基金重大项目和面上项目 |
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| 摘 要： | 基于微流控二维电泳芯片(2D-EMC)的流路特点,建立等效电阻模型,以便给出各端电压的合理取值范围,成功实现微流控芯片二维电泳分离.经实验测定各微通道电阻,在各端电压合理取值范围内,通过电流测量调整(优化)电压,得到了一组优化的电压控制方案,在化学发光-2D-EMC系统中成功实现了精氨酸和甘氨酸衍生物的二维分离.本方法显著减小了实现微流控芯片二维电泳分离的实验次数.
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| 关 键 词： | 微流控二维电泳芯片 等效电阻模型 电压控制 |
Optimal Control of Voltages on Two-dimensional Electrophoresis Microchip |
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| LIANG Heng,FAN Jun,LI Juan,SHEN Yan.Optimal Control of Voltages on Two-dimensional Electrophoresis Microchip[J].Chinese Journal of Analytical Chemistry,2009,37(10). |
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| Authors: | LIANG Heng FAN Jun LI Juan SHEN Yan |
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| Institution: | LIANG Heng,FAN Jun,LI Juan,SHEN Yan(Separation Science Institute,the Key Laboratory of Biomedical Information Engineering of Ministry of Education,Xi'an Jiaotong University,Xi'an 710049) |
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| Abstract: | Based on the microflow characteristics of two-dimensional electrophoresis microchip(2D-EMC),an equivalent resistance model of 2D-EMC was constructed to obtain the rational ranges of the voltages applied in each reservoir,and to implement the efficient 2D-EMC separations on microchips.With the experimental determinations of resistance of each micro-channel and the rational ranges of the applied voltages,additionally,through the current measurement-voltage adjustment,the optimal control scheme of the applied ... |
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| Keywords: | Two dimensional electrophoresis microchip equivalent resistance model voltage control |
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