| 糖胺聚糖的荧光标记及其与硫酸软骨素抗体的相互作用 |
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| 引用本文： | 韩章润,王玉峰,刘鑫,吴建东,曹欢,赵峡,柴文刚,于广利.糖胺聚糖的荧光标记及其与硫酸软骨素抗体的相互作用[J].分析化学,2011(9):1352-1357. |
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| 作者姓名： | 韩章润 王玉峰 刘鑫 吴建东 曹欢 赵峡 柴文刚 于广利 |
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| 作者单位： | 海洋药物教育部重点实验室;山东省糖科学与糖工程重点实验室;中国海洋大学;Glycosciences Laboratory;Faculty of Medicine;Imperial College London;Harrow;Middx HA1 3UJ; |
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| 基金项目： | 国家自然科学基金(Nos.31070724、30870506); 长江学者和创新团队发展计划(No.IRT0944); 国家海洋局公益专项(No.201005024)资助 |
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| 摘 要： | 以1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺和N-羟基琥珀酰亚胺为催化剂,将糖胺聚糖(肝素、硫酸乙酰肝素、硫酸软骨素A,B,C,D,E)中糖醛酸的羧基与氨基荧光素中氨基偶联,分别得到各荧光标记糖胺聚糖.将标记糖胺聚糖用点样仪点在硝酸纤维素包被玻璃芯片上,分别探讨其在硝酸纤维素薄膜上的保留率及其与硫酸软骨素抗体(克隆...
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| 关 键 词： | 糖胺聚糖 荧光标记 糖芯片 硫酸软骨素抗体 |
Fluorescent Labeling of Several Gycosaminoglycans and Their Interaction with Anti-Chondroitin Sulfate Antibody |
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| HAN Zhang-Run,WANG Yu-Feng,LIU Xin,WU Jian-Dong,CAO Huan,ZHAO Xia,CHAI Wen-Gang,YU Guang-Li.Fluorescent Labeling of Several Gycosaminoglycans and Their Interaction with Anti-Chondroitin Sulfate Antibody[J].Chinese Journal of Analytical Chemistry,2011(9):1352-1357. |
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| Authors: | HAN Zhang-Run WANG Yu-Feng LIU Xin WU Jian-Dong CAO Huan ZHAO Xia CHAI Wen-Gang YU Guang-Li |
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| Institution: | HAN Zhang-Run1,WANG Yu-Feng1,LIU Xin1,WU Jian-Dong1,CAO Huan1,ZHAO Xia1,CHAI Wen-Gang1,2,YU Guang-Li1 1(Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology,Ocean University of China,Qingdao 266003) 2(Glycosciences Laboratory,Faculty of Medicine,Imperial College London,Harrow,Middx HA1 3UJ,U.K) |
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| Abstract: | The amino groups of aminofluorescein were covalently linked to the carboxyl groups of glycosaminoglycans(GAGs)(Heparin,Heparan sulfate,Chondroitin sulfate A,B,C,D and E) using 1-ethyl-3-(1,3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide as catalytic reagent,and the fluorescent labeled glycosaminoglycan was acquired,separately.The fluorescent labeled GAGs were printed on the nitrocellulose coated glass chips with a manual microarrayer.The retention rate of GAGs on nitrocellulose membrane was cal... |
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| Keywords: | Glycosaminoglycans Fluorescent labeling Polysaccharide chip Monoclonal anti-chondroitin sulfate antibody |
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