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用基质辅助激光解吸电离飞行时间质谱法测定钙调蛋白的纯度与分子量
引用本文:季怡萍,佘益民,张杰,刘晓播,刘淑莹.用基质辅助激光解吸电离飞行时间质谱法测定钙调蛋白的纯度与分子量[J].分析化学,1999,27(7):814-816.
作者姓名:季怡萍  佘益民  张杰  刘晓播  刘淑莹
作者单位:中国科学院长春应用化学研究所,长春,130022
摘    要:采用基质辅助激光解吸电离飞行时间质谱法测定了钙调蛋白的纯度与分子量,并对所得的结果进行了讨论,实验结果表明本方法具有灵敏度高,分析速度快,重复性好,信息直观等特点,是其他传统测定蛋白质分子量的方法无法比拟的。

关 键 词:基质辅助激光解吸电离飞行时间质谱  钙调蛋白

Determination of Purity and Molecular Weight of Calmodulin by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Ji Yiping,She Yimin,Zhang Jie,Liu Xiaobo,Liu Shuying.Determination of Purity and Molecular Weight of Calmodulin by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,1999,27(7):814-816.
Authors:Ji Yiping  She Yimin  Zhang Jie  Liu Xiaobo  Liu Shuying
Abstract:The purity and molecular weight of calmodulin have been determined by means of matrix-assisted laser desorption/ionization time of flight mass spectrometry, and the results have been discussed, The experimental results demonstrate that this method is high sensitive and rapid as compared with other traditional methods.
Keywords:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry  calmodulin
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