| 微柱液相色谱和电化学检测法测定鼠脑微透析液中神经传递物质 |
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| 引用本文： | 黄铁华.微柱液相色谱和电化学检测法测定鼠脑微透析液中神经传递物质[J].分析化学,1998,26(6):748-751. |
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| 作者姓名： | 黄铁华 |
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| 作者单位： | BioanalyticalSystems!Inc，W，Lafayette，IN47906，USA，BioanalyticalSystemsInc!W，Lafayette，IN47906，USA |
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| 摘 要： | 报道了使用微柱色谱和电化学检测器测定鼠脑微透析液中神经传递物质的方法。儿茶酚胺，５－羟色胺和代谢物的检测限为 ０． １～０． ５ ｐｇ。含有乙酰胆碱酯酶和胆碱氧化酶的柱后反应器使分离后的乙酰胆碱和胆碱转化成过氧化氢．再用铂电极或过氧化物酶修饰过的玻碳电极检测过氧化氢。乙酰胆碱的检测限当使用怕电极时是５０× １０－１５ｍｏｌ，当使用酶修饰电极时是１０×１０－１５ｍｏｌ。
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| 关 键 词： | 神经递质 分析 儿茶酚胺 乙酰胆碱 5－羟色胺 |
Determination of Neurotransmitters in Rat Brain Microdialysates by Microbore Liquid Chromatography /Electrochemistry |
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| Huang Tiehua,Kissinger P T.Determination of Neurotransmitters in Rat Brain Microdialysates by Microbore Liquid Chromatography /Electrochemistry[J].Chinese Journal of Analytical Chemistry,1998,26(6):748-751. |
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| Authors: | Huang Tiehua Kissinger P T |
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| Abstract: | Determinations of neurotransmitters in rat brain microdialysates using microbore liquid chromatography with electrochemical detection are described. Catecholamines, serotonin and their metabolites in microdialysates were sensitively measured with detection limits of 0. 1 to 0. 5 pg per injection. Acetylcholine and choline were measured by using a post column immobilized enzyme reactor containing acetylcholinesterase and choline oxidase. The generated hydrogen peroxide was measured by using a platinum electrode or a peroxidase-re- dox polymer modified glassy carbon electrode. The acetylcholine detection limits were 50 f moles(injected) when using the Pt electrode and 10 f moles using the enzyme modified electrode.. |
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| Keywords: | Microbore liquid chromatography electrochemical detection neurotransmitters microdialysate |
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