| 二甲氧基硫代磷酸酯类农药多残留免疫分析方法研究 |
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| 引用本文： | 李永祥,徐振林,王弘,雷红涛,孙远明,沈丹,杨金易,沈玉栋.二甲氧基硫代磷酸酯类农药多残留免疫分析方法研究[J].分析化学,2010,38(11). |
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| 作者姓名： | 李永祥 徐振林 王弘 雷红涛 孙远明 沈丹 杨金易 沈玉栋 |
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| 作者单位： | 1. 华南农业大学食品学院,广东省食品质量安全重点实验室,广州,510642
2. 广州市动物卫生监督所,广州,510440 |
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| 基金项目： | 国家自然科学基金(No.30901005); 国家863计划(No.2007AA10Z437); 广东省科技计划项目(Nos.2009A020101004,2009B011300005); 华南农业大学新学科扶持基金(No.5100-K08198)资助项目 |
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| 摘 要： | 以二甲氧基硫代磷酸酯类农药为目标,设计合成了系列半抗原及抗原,制备了4种宽谱特异性抗体。研究结果表明,含不饱和烷烃手臂的半抗原所制备的抗体宽谱特异性优于含酰胺键手臂的半抗原所制备的抗体。采用目标待测物的特征次级结构作为包被半抗原可显著提高ELISA检测灵敏度。经条件优化建立的最佳间接竞争ELISA多残留检测方法可同时检测8种常用高毒农药,其检出限(LOD)在2.6～104μg/kg之间,符合相关限量标准要求。生菜样品药物添加平均回收率为73.9%～121.4%;平均相对标准偏差为10.6%～18.4%。菜心样品药物添加平均回收率为80.4%～121.2%;平均相对标准偏差为13.5%～24.4%。方法精密度均达到气相色谱法的检测水平。
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| 关 键 词： | 有机磷农药 多残留检测 异源包被 酶联免疫吸附分析 |
Development of Broad-specificity Immunoassay for Detection of O,O-Dimethyl Organophosphorus Pesticides |
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| LI Yong-Xiang,XU Zhen-Lin,WANG Hong,LEI Hong-Tao,SUN Yun-Ming,SHEN Dan,YANG Jin-Yi,SHEN Yu-Dong.Development of Broad-specificity Immunoassay for Detection of O,O-Dimethyl Organophosphorus Pesticides[J].Chinese Journal of Analytical Chemistry,2010,38(11). |
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| Authors: | LI Yong-Xiang XU Zhen-Lin WANG Hong LEI Hong-Tao SUN Yun-Ming SHEN Dan YANG Jin-Yi SHEN Yu-Dong |
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| Institution: | LI Yong-Xiang1,XU Zhen-Lin1,WANG Hong1,LEI Hong-Tao1,SUN Yun-Ming1,SHEN Dan2,YANG Jin-Yi1,SHEN Yu-Dong* 2 1 ( College of Food Science,South China Agricultural University,Guangzhou 510642) 2 ( Guangzhou Animal Health Inspection Institute,Guangzhou 510440) |
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| Abstract: | Taking O,O-dimethyl organophosphorus pesticides as targets,a series of haptens were designed and synthesized. Four antibodies with broad-specificity to O,O-dimethyl organophosphorus pesticides were produced. Results showed that the broad specificity of antibody produced by hapten with unsaturated alkane arm was better than that of the haptens with amide bond on the benzene ring. The sensitivity of ELISA to O,O-dimethyl organophosphorus pesticides could be significantly improved by using the coating antigen ... |
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| Keywords: | Organophosphorus pesticides Broad-specificity Heterology coating Enzyme-linked immunosorbent assay |
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