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尼古丁对乳酸脱氢酶活性的影响
引用本文:甘震海,庄乾坤,张建勋.尼古丁对乳酸脱氢酶活性的影响[J].分析化学,2002,30(4):385-387.
作者姓名:甘震海  庄乾坤  张建勋
作者单位:1. 北京大学化学学院,北京,100871
2. 河南新郑卷烟厂,新郑,451150
基金项目:国家自然科学基金 (No .2 0 0 0 5 0 0 1),教育部回国人员启动基金资助项目
摘    要:用计时电流法成功地研究了尼古丁对乳酸脱氢酶活性的影响,测定了尼古丁存在与否对乳酸脱氢酶酶促反应的初速度V0、酶促反应最大反应初速度Vm及米氏常数Km。实验结果表明尼古丁对乳酸脱氢酶的活性有很大影响,但在高浓度的NADH存在下,尼古丁对乳酸脱氢酶活性的影响将得到大大降低。

关 键 词:计时电流  乳酸脱氢酶  尼古丁  酶活性  生物碱  烟草  相互作用

Investigation of the Effect of Nicotine on the Activity of Lactate Dehydrogenase
Gan Zhenhai,Zhuang Qiankun.Investigation of the Effect of Nicotine on the Activity of Lactate Dehydrogenase[J].Chinese Journal of Analytical Chemistry,2002,30(4):385-387.
Authors:Gan Zhenhai  Zhuang Qiankun
Institution:Gan Zhenhai,Zhuang Qiankun *
Abstract:Chronoamperometry based on the "controlling diffusion layer" concept of the convective system was used to monitor the effect of nicotine on the activity of lactate dehydrogenase (LDH) at a bare glassy carbon (GC) electrode. The real initial reaction rate ( V 0) and the maximum reaction rate ( V max ) of the enzyme catalyzed reaction and the Michaelis Menten constant ( K m) were determined. Analysis of the experimental results revealed that the nicotine ion could inhibit the LDH activity remarkably. But, when the concentration of nicotinamide adenine dinucleotide (NADH) was high, the inhibition would be decreased deeply.
Keywords:Chronoamperometry  lactate dehydrogenase  nicotine  enzyme activity  
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