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离子液体保护聚苯胺纳米金复合膜传感器的制备及其在检测乳制品中金黄色葡萄球菌毒素B的应用
引用本文:孙秀兰,高博,张银志.离子液体保护聚苯胺纳米金复合膜传感器的制备及其在检测乳制品中金黄色葡萄球菌毒素B的应用[J].分析化学,2012(7):1086-1091.
作者姓名:孙秀兰  高博  张银志
作者单位:江南大学食品学院;江南大学食品科学与技术国家重点实验室
基金项目:国家自然科学基金项目(No.20806033);张家港市科技局项目(No.ZKS0904);中央高校基本科研业务费专项资金项目(No.JUSRP21127)资助
摘    要:采用原位合成法制得聚苯胺修饰纳米金的复合膜,并采用紫外、红外及透射电镜对其进行表征,以离子液体1,3-二丁基-咪唑六氟磷酸盐为保护复合膜的溶剂,成功制得离子液体保护的聚苯胺纳米金复合膜免疫传感器,并应用于乳品中金黄色葡萄球菌肠毒素B(SEB)的检测。在Fe(CN)63-/4-溶液中,采用循环伏安法(CV)及交流阻抗法(EIS)对传感器进行表征与测定,建立了SEB检测标准曲线,Y=933.46x+1399.8,线性范围为0.1~8.0μg/L,相关系数R2=0.9932,检出限明显降低(0.033μg/L,S/N=3)。结果表明,本传感器特异性良好,乳品检测回收率在88%~119%之间,稳定性好,可应用于乳品中的SEB快速检测。

关 键 词:免疫传感器  乳制品  金黄色葡萄球菌肠毒素B  聚苯胺纳米金修饰膜  交流阻抗法  离子液体

An Electrochemical Immunosensor Modified with Polyaniline-Gold Composite Film for Detecting Staphylococcus Aureus Enterotoxin B in Dairy Products
SUN Xiu-Lan,GAO Bo,ZHANG Yin-Zhi.An Electrochemical Immunosensor Modified with Polyaniline-Gold Composite Film for Detecting Staphylococcus Aureus Enterotoxin B in Dairy Products[J].Chinese Journal of Analytical Chemistry,2012(7):1086-1091.
Authors:SUN Xiu-Lan  GAO Bo  ZHANG Yin-Zhi
Institution:1(School of Food Science,Jiangnan University,WuXi 214122,China) 2(The Key Laboratory of Food Science and Technology,Jiangnan University,WuXi 214122,China)
Abstract:taphylococcus aureus enterotoxin B(SEB) is a kind of extracellular toxins that is secreted by Staphylococcus aureus and mainly existed in high protein foods,for example,milk.In this study,an immunosensor modified with polyaniline-gold composite film was prepared and its characteristics such as favorable conductivity of the material,high stability due to the protective effect of ionic liquid and lower detection limit were also investigated.The sensor was characterized and detected by cyclic voltammetry and electrochemical impedance spectroscopy in the Fe(CN) 6 3-/4- solution.The relevant conditions was optimized,and the SEB standard curve,Y=933.46x+1399.8,was established.The linear range was 0.1-8μg / L with a correlation coefficient of 0.9932,and the detection limit was 0.033μg / L(S / N=3).The sensor showed good specificity,dairy detection rate between 88%119%,renewable use,stability,and has been used for the rapid detection of SEB in dairy products.
Keywords:Immunosensor  Dairy product  Staphylococcus aureus enterotoxin B  Polyaniline-gold composite film  Electrochemical impedance spectroscopy  Ionic liquid
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