三相中空纤维膜液相微萃取-高效液相色谱法测定水中痕量双酚A

建立了三相中空纤维膜液相微萃取-高效液相色谱(HF-LPME-HPLC)方法,用于分析测定水中痕量双酚A的含量.设计了三相中空纤维膜液相微萃取系统,优化的HP-LPME最佳萃取条件为:萃取剂为正辛醇,接受相NaOH浓度为0.09 mol/L,样品溶液pH=4.0,NaC1加入量为30 g/L,搅拌速度为900 r/min,萃取时间为60 min.萃取后取20 μL接受相进行色谱分析.在最佳萃取条件下,方法的线性范围为0.5～200 μg/L(r＞ 0.999),检出限(信噪比为3)为0.2 μg/L;富集因子为241；方法RSD＜3.2％ (n=3).在实际环境水样中添加5,20和50μg/L的双酚A标准物质,加标平均回收率为92.8％～101.9％.表明本方法可用于水中痕量双酚A的快速准确测定.

Determination of Trace Bisphenol-A in Water Using Three-phase Hollow Fiber Liquid Phase Microextraction Coupled with High Performance Liquid Chromatography

A method for the determination of trace bisphenol-A(BPA) in water was developed by HPLC using three-phase hollow fiber liquid phase microextraction as sample preparation technique.The system of three-phase hollow fiber liquid phase microextraction was designed.The optimized microextraction conditions were as follows: 1-octanol was used as the extraction solvent,the concentration of NaOH solution as acceptor phase was 0.09 mol/L,the pH value of sample solutions was 4.0,the NaCl concentration in sample solutions was 30 g/L,the stirring rate was 900 r/min and the extraction time was 60 min.The determination of BPA was finished with injecting 20 μL acceptor phase by HPLC.Under the optimal microextraction conditions,the linear range for BPA was 0.5-200 μg/L(r>0.999),the detection limits(S/N=3) of BPA was 0.2 μg/L,the enrichment factor was 241.1,and the relative standard deviations(RSD) was lower than 3.2%.The proposed method was applied to the determination of the BPA in environmental water samples.The spiked average recoveries were within the range of 92.8%-101.9%(n=3).The developed method is simple and environmentally friendly for the determination of BPA in water.