| 顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物 |
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| 引用本文： | 肖天鹏,郑晓峰,吕吉鸿,郭泽峰,高峰,刘春凤,李崎.顶空-固相微萃取-气相色谱法检测可可麦汁中吡嗪类化合物[J].分析化学,2012,40(10):1589-1592. |
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| 作者姓名： | 肖天鹏 郑晓峰 吕吉鸿 郭泽峰 高峰 刘春凤 李崎 |
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| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,无锡214122;江南大学酿酒科学与工程研究室,无锡214122
2. 杭州千岛湖啤酒有限公司,淳安,311700 |
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| 基金项目： | 国家“十一五”支撑重点项目(No.2008BAI63B06)资助 |
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| 摘 要： | 建立了顶空-固相微萃取(HS-SPME)-气相色谱快速测定可可麦汁中3种吡嗪类物质(2,5-二甲基吡嗪、2,3,5-三甲基吡嗪和2,3,5,6-四甲基吡嗪)的方法.选择不同的固相微萃取头对萃取温度和时间进行优化,所得最佳萃取条件为:在60℃下,采用75 μm CAR/PDMS萃取头对麦汁样品萃取40 min.本方法的检出限(S/N=3)为0.023～ 0.056 μg/L,线性范围1～500 mg/L;相对标准偏差为3.6％～6.4％；回收率为95.4％～102.7％.本方法应用于样品检测,发现可可麦汁中吡嗪的浓度与原料中可可粉的添加量正相关,显示了很好灵敏性.
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| 关 键 词： | 顶空-固相微萃取(HS-SPME) 吡嗪类物质 气相色谱 可可麦汁 |
Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction |
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| XIAO Tian-Peng
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ZHENG Xiao-Feng
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L Ji-Hong
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GUO Ze-Feng
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GAO Feng
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LIU Chun-Feng
,
LI Qi.Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction[J].Chinese Journal of Analytical Chemistry,2012,40(10):1589-1592. |
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| Authors: | XIAO Tian-Peng ZHENG Xiao-Feng L Ji-Hong GUO Ze-Feng GAO Feng LIU Chun-Feng LI Qi |
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| Institution: | 1,2 1(The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,China) 2(Lab of Brewing Science and Technology,School of Biotechnology, Jiangnan University,Wuxi 214122,China) 3(Hangzhou Qiandaohu Brewery Co.Ltd,Chun-an 311700,China) |
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| Abstract: | A headspace solid phase microextraction(HS-SPME),followed by gas chromatographic method was established for the detection of three pyrazine compounds(2,5-dimethyl pyrazine,2,3,5-trimethyl pyrazine and 2,3,5,6-tetramethyl pyrazine) in cocoa wort.Equilibrium temperature and equilibrium time were optimized by using different SPME heads.According to the results of the optimization experiments,the following conclusion can be drawn:The cocoa wort sample was efficiently extracted under 40 ℃ for 40 min with 75 μm CAR/PDMS SPME head.The method had linear response within 1-500 mg/L,and the detection limits(S/N=3) were below 0.023 μg/L.The relative standard deviations were from 3.6%-6.4% and the recoveries were from 95.4% to 102.7%.This method is characterized by rapidly,high sensitivity,good linearity and repeatability for the analysis of cocoa wort samples. |
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| Keywords: | Headspace solid phase microextraction Pyrazine compounds Gas chromatography Cocoa wort |
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