| 微囊藻毒素分子印迹传感器的制备与应用 |
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| 引用本文： | 申晴,崔莉凤,赵硕,李科.微囊藻毒素分子印迹传感器的制备与应用[J].分析化学,2012(3):442-446. |
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| 作者姓名： | 申晴 崔莉凤 赵硕 李科 |
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| 作者单位： | 北京工商大学食品学院;河北联合大学轻工业学院 |
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| 基金项目： | 北京市自然科学基金(No.2102015)资助项目 |
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| 摘 要： | 以邻氨基酚为单体,微囊藻毒素(MC-LR)为模板,采用循环伏安法在金电极的表面电聚合成膜分子印迹材料,制备了传感器。采用安培法对MC-LR进行检测。在制备影响条件最佳值(pH=4.5;单体/模板=1.4×108∶1;洗脱时间10 min)的基础上,对该传感器的线性范围、使用寿命、选择性等进行了研究,并与液相色谱方法进行对比,结果表明:该传感器对MC-LR具有良好的选择性和灵敏度,线性范围为0.05～0.35 mg/L;加标回收率为80%～105%;检出限为7.3μg/L。与液相色谱方法对比,当置信度为99%时,无系统误差。
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| 关 键 词： | 微囊藻毒素 分子印迹 电聚合 传感器 |
Preparation and Application of Microcystins Sensor Based on Molecular Imprinting |
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| SHEN Qing,CUI Li-Feng,ZHAO Shuo,LI Ke.Preparation and Application of Microcystins Sensor Based on Molecular Imprinting[J].Chinese Journal of Analytical Chemistry,2012(3):442-446. |
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| Authors: | SHEN Qing CUI Li-Feng ZHAO Shuo LI Ke |
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| Institution: | 1(Food institute,Beijing Technology and Business University,Beijing 100048,China) 2(Qinggong College,Hebei United University,Tangshan 603000,China) |
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| Abstract: | A molecularly imprinted film sensor was prepared by cyclic voltammetry on a gold electrode surface using O-aminophenol as the monomer,microcystin(MC-LR) as a template,and then MC-LR was determined by amperometry.The optimum preparation conditions for the sensor were as follows: pH=4.5,monomer/template=1.4×108 ∶ 1;elution time=10 min.The characteristics of the sensor such as the linear relationship,service life,selectivity were investigated.Experimental results showed that the linear response of the sensor is 0.05-0.35 mg/L,the recovery of standard addition is 80% to 105% and the detection limit is 7.3 μg/L.In comparison with HPLC,when the confidence level was 99%,there is no systematic error.The sensor exhibited a good selectivity and sensitivity |
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| Keywords: | Microcystins Molecular imprinting Electricity polymerization Sensor |
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