| 液相色谱-串联质谱法测定鸡组织中沃尼妙林残留 |
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| 引用本文： | 王蕊,远立国,贺利民,朱理想,罗显阳,刘雅红.液相色谱-串联质谱法测定鸡组织中沃尼妙林残留[J].分析化学,2010,38(7). |
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| 作者姓名： | 王蕊 远立国 贺利民 朱理想 罗显阳 刘雅红 |
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| 作者单位： | 华南农业大学兽医学院,广州,510642 |
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| 基金项目： | "十一五"国家科技支撑计划课题 |
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| 摘 要： | 建立了鸡组织中沃尼妙林残留的液相色谱-电喷雾串联质谱(LC-ESI-MS /MS) 检测方法.样品用乙腈提取后,过Strata-X-C固相萃取小柱净化.以乙腈-0.1%甲酸水溶液(35∶ 65,V/V)为流动相,经Luna C18色谱柱分离,采用电喷雾电离,多反应监测(MRM)正离子模式对沃尼妙林进行定性与定量分析.采用基质匹配法,对鸡皮肤、肌肉、肾脏和肝脏中沃尼妙林的含量进行标准校正,在5(7.5)～500 μg/kg范围内线性良好(r>0 998),在鸡肝脏中的检出限(LOD)及定量限(LOQ)分别为4.0和7.5 μg/kg;其它3种组织中的LOD及LOQ分别为2.5和5.0 μg/kg.在5(7.5), 50及500 μg/kg添加水平下,4中组织中沃尼妙林的平均回收率为78 5%～104.0%.日内相对标准偏差(RSD)为1.2%～9.8%,日间相对标准偏差为3.7%～13.2%.
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| 关 键 词： | 沃尼妙林 鸡组织 残留 液相色谱-串联质谱 |
Determination of Valnemulin Residues in Chicken Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| WANG Rui,YUAN Li-Guo,HE Li-Min,ZHU Li-Xiang,LUO Xian-Yang,LIU Ya-Hong.Determination of Valnemulin Residues in Chicken Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(7). |
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| Authors: | WANG Rui YUAN Li-Guo HE Li-Min ZHU Li-Xiang LUO Xian-Yang LIU Ya-Hong |
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| Institution: | WANG Rui,YUAN Li-Guo,HE Li-Min,ZHU Li-Xiang,LUO Xian-Yang,LIU Ya-Hong (Veterinary Medicine College of South China Agricultural University,Guangzhou 510642) |
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| Abstract: | A liquid chromatography with electrospray ionization tandem mass spectrometry detection (LC ESI MS/MS) method for the determination of valnemulin residues in chicken tissues was developed.The analyte was extracted with acetonitrile and the extract was cleaned up by the Strata-X-C solid phase extraction column.Chromatographic separation of valnemulin was performed in isocratic mode,on a C18 column using acetonitrile and 0.1% formic acid aqueous solution (35:65,V/V).Qualitation and quantification of valnemuli... |
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| Keywords: | Valnemulin Chicken tissues Residue Liquid chromatography-tandem mass spectrometry |
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