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| 生物合成咖啡酸苯乙酯体系的液相色谱-串联质谱快速分析 | |
| 引用本文： | 卢定强,蒋奔,王俊,赵辉,凌岫泉,柴宏,夏亭.生物合成咖啡酸苯乙酯体系的液相色谱-串联质谱快速分析[J].分析化学,2010,38(11). |
| 作者姓名： | 卢定强 蒋奔 王俊 赵辉 凌岫泉 柴宏 夏亭 |
| 作者单位： | 1. 南京工业大学药学院,南京,210009;江苏省药物研究所,南京,210009 2. 南京工业大学药学院,南京,210009 3. 南京工业大学药学院,南京,210009;江苏科技大学生物与环境工程学院,镇江,212018 4. 江苏科技大学生物与环境工程学院,镇江,212018 |
| 基金项目： | 国家自然科学基金(No.20876076); 国家重点基础研究发展计划(No.2009CB724700); 江苏省自然科学基金(No.BK2009213)资助项目 |
| 摘 要： | 建立液相色谱-串联质谱(LC-MS/MS)检测生物合成咖啡酸苯乙酯体系中咖啡酸(Caffeic acid,CA)和咖啡酸苯乙酯(Caffeic acid phenethylester,CAPE)的方法。采用LC-MS/MS电喷雾电离(ESI),负离子选择反应监测(SRM)模式检测。以V(乙腈)∶V(水)∶V(冰醋酸)=55∶45∶0.5为流动相,流速1.0mL/min,Hypersil C18色谱柱分离并检测生物合成咖啡酸苯乙酯体系中的咖啡酸以及咖啡酸苯乙酯的含量,并对生物合成咖啡酸苯乙酯的收率进行了动力学分析。本方法在进样量为0.2～20μg时具有良好的线性关系,咖啡酸和咖啡酸苯乙酯样品的加标回收率分别为93.4%～98.2%和90.3%～97.8%,相对标准偏差分别为1.79%～2.56%和1.82%～3.67%,咖啡酸苯乙酯收率在3d内可以达到15.54%,表明本方法简便、快速、可靠。 |
| 关 键 词： | 液相色谱-串联质谱法 咖啡酸苯乙酯 咖啡酸 生物(酶)合成体系 |
Rapid Determination of Enzymatic Synthesis Caffeic Acid Phenethyl Ester in an Enzymecatalytic and Synthetic System by Liquid Chromatography-Tandem Mass Spectrometry |
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| LU Ding-Qiang,JIANG Ben,WANG Jun,ZHAO Hui,LING Xiu-Quan,CHAI Hong,XIA Ting.Rapid Determination of Enzymatic Synthesis Caffeic Acid Phenethyl Ester in an Enzymecatalytic and Synthetic System by Liquid Chromatography-Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(11). | |
| Authors: | LU Ding-Qiang JIANG Ben WANG Jun ZHAO Hui LING Xiu-Quan CHAI Hong XIA Ting |
| Institution: | LU Ding-Qiang* 1,2,JIANG Ben1,WANG Jun1,3,ZHAO Hui1,LING Xiu-Quan1,CHAI Hong1,XIA Ting3 1 ( School of Pharmaceutical Sciences,Nanjing University of Technology,Nanjing 210009) 2 ( Jiangsu Provincial Institute of Materia Medica,Nanjing 210009) 3 ( College of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang 212018) |
| Abstract: | A liquid chromatography-tandem mass spectrometric method was established for the simultaneous determination of caffeic acid ( CA) and caffeic acid phenethyl ester ( CAPE) in enzymatic synthesis,and the biosynthesis kinetics of caffeic acid phenethyl ester was analyzed. Identification was carried out by electrospray ionization ( ESI) in negative mode using selected reaction monitoring. CA and CAPE in enzymatic synthesis system were separated on a hypersil C18 column. The mobile phase consisted of acetonitril... |
| Keywords: | Liquid chromatography-tandem mass spectrometry Caffeic acid phenethyl ester Caffeic acid Enzymatic synthesis |
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