| 流动注射化学发光法测定尿酸 |
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| 引用本文： | 张道建,黄玉明,章竹君.流动注射化学发光法测定尿酸[J].分析化学,2001,29(4):446-449. |
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| 作者姓名： | 张道建 黄玉明 章竹君 |
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| 作者单位： | 西南师范大学分析科学研究所, |
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| 基金项目： | 国家自然科学基金，重庆市自然科学基金资助课题 |
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| 摘 要： | 在碱性条件下，铁氰化钾氧化鲁米诺，产生化学发光，尿酸对该体系的化学发光有显著的增强作用（亚铁氰化钾存在时）。基于此，建立了一种直接测定尿酸的流动注射化学发光分析法。方法的线性范围为２．０×１０－８～５．０×１０－６ ｇ／ｍＬ；检测限（３σ）为６．７×１０－９ ｇ／ｍＬ；相对标准偏差为１．１％（尿酸１．０×１０－７ ｇ／ｍＬ，ｎ＝１１）。用于血清及尿样中尿酸的分析，结果令人满意。
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| 关 键 词： | 流动注射分析 化学发光 尿酸 |
| 修稿时间： | 2000年6月7日 |
Flow Injection Chemiluminescence Determination of Uric Acid |
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| Zhang Daojian.Flow Injection Chemiluminescence Determination of Uric Acid[J].Chinese Journal of Analytical Chemistry,2001,29(4):446-449. |
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| Authors: | Zhang Daojian |
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| Abstract: | A flow injection chemiluminescence method for the determination of uric acid was described. The method is based on the chemiluminescence produced during the oxidation of luminol by hexacyanoferrate *# in an alkaline solution. The emission intensity is greatly enhanced by uric acid in the presence of hexacyanoferrate *" as suppressor. The flow system is very stable. the proposed method is simple, sensitive, and inexpensive. The chemiluminescence intensity is proportional to uric acid concentration over the range 2.0×10－8～5．0×10－6 g/mL with a detection limit of 6.7×10－9 g/mL. The relative standard deviation (RSD) in 1.1% (n=11). The method has been successfully applied to the determination of uric acid in human urine and serum samples. In addition, the possible reaction mechanism has been discussed as well. |
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| Keywords: | Chemiluminescence analysis flow injection uric acid |
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