| 圆二色光谱分析蛋白质构象的方法及研究进展 |
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| 引用本文： | 沈星灿,梁宏,何锡文,王新省.圆二色光谱分析蛋白质构象的方法及研究进展[J].分析化学,2004,32(3):388-394. |
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| 作者姓名： | 沈星灿 梁宏 何锡文 王新省 |
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| 作者单位： | 广西师范大学化学化工学院,生物无机研究所,桂林,541004;南开大学化学系,天津,300071;广西师范大学化学化工学院,生物无机研究所,桂林,541004;南开大学化学系,天津,300071 |
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| 基金项目： | 国家自然科学基金 (No .2 0 2 610 0 1)，广西自然科学基金 (桂科青 0 3 3 90 2 2 )，高校优秀青年教师奖励计划资助项目 |
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| 摘 要： | 介绍了远紫外圆二色光谱数据计算蛋白质二级结构，辨认蛋白质三级结构类型的原理、拟合方法、实验技术。对近紫外圆二色作为光谱探针，研究蛋白质中芳香氨基酸残基、二硫键微环境的变化作了简单介绍。
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| 关 键 词： | 圆二色 蛋白质二级结构 蛋白质三级结构 评述 |
Recent Trends and Spectroscopic Methods for Analysis of the Protein Conformation with Circular Dichroism |
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| Shen Xingcan ,Liang Hong ,He Xiwen,Wang Xinsheng.Recent Trends and Spectroscopic Methods for Analysis of the Protein Conformation with Circular Dichroism[J].Chinese Journal of Analytical Chemistry,2004,32(3):388-394. |
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| Authors: | Shen Xingcan Liang Hong He Xiwen Wang Xinsheng |
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| Institution: | Shen Xingcan 1,2,Liang Hong *1,He Xiwen2,Wang Xinsheng2 1 |
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| Abstract: | The principles, methods, and experimental techniques for the determination of the protein secondary structures and identification of tertiary structures with far-ultraviolet (UV) circular dichroism (CD) data are reviewed. The method that near-UV CD spectrum served as useful probes to provide the information of dissymmetric environments of aromatic residues and disulfur bonds is also introduced briefly. |
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| Keywords: | Circular dichroism protein secondary structure protein tertiary structure review |
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