基于NMR的代谢组学方法对硝酸钕急性生物效应的研究

采用现代核磁共振和模式识别技术,分析了腹腔注射给药2、10和50mg/kg体重剂量硝酸钕48h内Wistar大鼠尿液和血清的核磁共振氢谱。由尿液及血清中内源性代谢物如柠檬酸、肌酸酐、N-氧三甲胺、氨基酸、乳酸、琥珀酸、牛磺酸及葡萄糖等物种的浓度变化,结合大鼠血清指标和肝、肾组织切片图研究了轻稀土化合物Nd(NO3)3在大鼠体内的急性生物效应。结果表明,3个剂量的Nd(NO3)3主要对大鼠肝脏造成了不同程度的损伤,而且随着剂量的升高渐趋严重。同时,Nd(NO3)3也对肾脏的特定部位(肾乳头、肾小管)造成了损害。

Studies on the Acute Biochemical Effects of Nd(NO3)3 by Nuclear Magnetic Resonance-based Metabonomics

The biochemical effects of Nd(NO3)3 was stud ied by NMR-based metabonomics.Male Wistar rats were administrated intraperiton eally with Nd(NO3)3 at doses of 2,10 and 50 mg/kg body weight.Urine was co llected over a 48-h period after dosing and serum was collected after 48 h.1H NMR spectra of biofluids(urine and serum)were analyzed by pattern recognition using principal components analysis.The studies showed t hat there was a dose-related biochemical effects of Nd(NO3)3 treatment.The liver and kidney(renal tubular and renal papilla)damage induced by Nd(NO 3)3 was characterized by the variations of many endogenous metabolites,such as citrate,creatinine,N-trimethyl dioxide,dimethyl amine,dimethyl glyc ine,lactate,taurine and glucose.The NMR-based metabonomics can be applied to study the biochemical effects or toxicities of other compounds.