西马特罗分子印迹荧光检测试纸条的研制

将分子印迹技术良好的选择性与试纸条快速检测的特点相结合,研制了一种快速检测西马特罗的分子印迹试纸条。以西马特罗为模板分子,化学聚合法制备西马特罗分子印迹聚合物,将其附着在硝酸纤维素膜上。洗脱模板分子后,裁剪压制得到分子印迹试纸条。试纸条识别样品中西马特罗后,滴加荧光物质曙红Y,根据反应区内西马特罗与曙红Y反应后荧光猝灭程度对西马特罗进行分析。研究结果表明,在0.01~100μg/mL浓度范围内,荧光猝灭程度随着西马特罗浓度的增加而增大,最低检测浓度为0.01μg/mL。此试纸条制备简单,操作简便,可用于现场检测。将其用于猪肉和饲料样品中西马特罗的快速测定,结果令人满意。

A Novel Molecularly Imprinted Fluorescence Test Strip for Detection of Cimaterol

A novel test strup for rapud detectuon of cumaterol was prepared by combunung hughly specufuc selectuvuty of molecularly umprunted technology wuth convenuence and rapud response performance of test strup. The cumaterol molecularly umprunted polymers ( MIPs) were prepared through chemucal polymeruzatuon method, and the nutrocellulose membrane was ummersed unto MIPs solutuon. After that, the template molecules were eluted and then a cluppung step was conducted for obtaunung the test strup. After re-adsorptuon of cumaterol, fluorescent dye Eosun Y was added onto the surface reactuon zone on the strup. Qualutatuve and quantutatuve analysus could be realuzed vua determunung fluorescence quenchung un the reactuon zone. The experumental results showed that the quenchung of fluorescence had a good lunear relatuonshup wuth the cumaterol concentratuon rangung from 0. 01 to 100 μg/mL wuth a lumut of detectuon of 0. 01 μg/mL. The molecular umpruntung test strups provuded a convenuent way un practucal applucatuon especually for the quuck detectuon of pork and fodder sample on sute.