高效液相色谱-电喷雾离子阱质谱法测定红葡萄酒中酚类物质

建立了高效液相色谱-质谱联用(HPLC-MS)技术检测葡萄酒中酚类物质的方法,并评价其定量分析的准确性、线性、重复性和检出限,结果显示:16种酚类物质除芦丁和白藜芦醇糖苷外,平均加标回收率大于78%;相关系数R2>0.999,线性关系良好;保留时间和峰面积在日内(n=10)与日间(n=6)重复性的相对标准偏差(RSD)均分别低于2.0%和5.0%;检出限为0.05~1.0 mg/L。利用该方法对6个红葡萄酒中16种酚类物质进行了定性定量分析,其相对标准偏差(CV%)均小于5.0%,说明本方法简便、准确、灵敏、重现性好,可用于葡萄酒产品质量监控。

Determination of Phenolic Compounds in Red Wines by Reversed Phase High Performance Liquid Chromatography and Electrospray Ion Trap Mass Spectrometry

A high performance liquid chromatography/electrospray ionization mass spectrometric method has been developed for the determination of phenolic compounds in red wines.This method was validated for accuracy,linearity,repeatability and limits of detection(LOD).The results show that average recoveries of 16 phenolic standards exceed 78.5% except for rutin and trans-piecid;linearity is good(R~2>0.999);the relative standard deviations of repeatability of inter-day(n=10) and intra-day(n=6) for retention time and peak area are less than 2.0% and 5.0%,respectively;LOD is 0.05-1.0 mg/L. 16(phenolic) compounds in 6 red wines were determined by the method established,and the relative standard deviation(%) for triplicate of each compound was less than 5.0%.The method was reliable for quality control of red wine.