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| 固相萃取-高效液相色谱-电喷雾串联质谱法分析芍药和酒芍药的水溶性化学成分 | |
| 引用本文： | 何忠梅,孙佳明,张辉,王华.固相萃取-高效液相色谱-电喷雾串联质谱法分析芍药和酒芍药的水溶性化学成分[J].分析化学,2009,37(8). |
| 作者姓名： | 何忠梅 孙佳明 张辉 王华 |
| 作者单位： | 1. 吉林农业大学中药材学院,长春,130116;长春中医药大学中医药与生物工程研究开发中心,长春,130117 2. 长春中医药大学中医药与生物工程研究开发中心,长春,130117 3. 哈尔滨商业大学药学院,哈尔滨,150000 |
| 摘 要： | 利用固相萃取-高效液相色谱-电喷雾串联质谱法(SPE-HPLC-DAD-ESI-MS2)分析了芍药(Paeonia lactiflora Pall.)及其炮制品酒芍药(Paeonia lactiflora Pall.preparated with wine)水煎液中化学成分的种类和结构,并对其主要有效成分芍药苷进行了定量测定.用此方法得到了芍药和酒芍药水煎液的紫外色谱(UV)图、总离子流色谱(TIC)图和萃取离子色谱(EIC)图,以及相应色谱峰的紫外光谱图和EIC/MS2的质谱图.对其进行解析,发现芍药和酒芍药水煎液中所含化学成分基本一致,但酒芍药水煎液中除主要活性成分芍药苷含量基本未变外,其它酚酸性化学成分含量相对于芍药水煎液均有不同程度的下降.同时鉴别出芍药和酒芍药水煎液中共同含有的16种成分,它们分别是:蔗糖、(+)-儿茶素、Mudanpioside E、芍药新苷、芍药内酯苷、氧化芍药苷、芍药苷、羟基芍药苷、没食子酰芍药苷*、牡丹皮苷Ⅰ、苯甲酰芍药苷、三没食子酰葡萄糖、四没食子酰葡萄糖、五没食子酰葡萄糖.以上分析说明,酒炙可保留芍药的药效,同时"缓和其酸寒伐肝之性". |
| 关 键 词： | 芍药 固相萃取 高效液相色谱 电喷雾串联质谱 水溶性 |
Analysis of Components in Aqueous of Paeonia Lactiflora Pall and Paeonia Lactiflora Pall Preparation with Wine by Solid Phase Extraction-High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry |
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| HE Zhong-Mei,SUN Jia-Ming,ZHANG Hui,WANG Hua.Analysis of Components in Aqueous of Paeonia Lactiflora Pall and Paeonia Lactiflora Pall Preparation with Wine by Solid Phase Extraction-High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(8). | |
| Authors: | HE Zhong-Mei SUN Jia-Ming ZHANG Hui WANG Hua |
| Institution: | HE Zhong-Mei1,2,SUN Jia-Ming2,ZHANG Hui2,WANG Hua3 1(College of Chinese Medicine Materials,Jilin Agricultural University,Changchun 130117)2(Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Traditional Chinese Medicine,Changchun 130117)3(College of Pharmaceutical Sciences,Harbin University of Commerce,Haerbin 150000) |
| Abstract: | A solid phase extraction and high performance liquid chromatography-electrospray ionization-mass spectrometry was developed for the analysis and identification of components in the aqueous of Paeonia lactiflora Pall and Paeonia lactiflora Pall preparation with wine.The ultraviolet chromatogram,total ion current chromatogram and extracted ion chromatogram of the samples and ESI/MS/MS spectra of the peaks in the chromatograms were obtained in order to identify the structure of compounds.It showed that there w... |
| Keywords: | Paeonia lactiflora Pall solid phase extraction high performance liquid chromatography electrospray ionization mass spectrometry water-soluble constituents |
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