| 糖和糖醇的高效液相色谱分析 |
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| 引用本文： | 徐秀珠,徐根良.糖和糖醇的高效液相色谱分析[J].分析化学,1999,27(5):547-550. |
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| 作者姓名： | 徐秀珠 徐根良 |
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| 作者单位： | 浙江大学应用化学研究所,杭州,310027 |
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| 摘 要： | 采用高效液相色谱示差折光检测器（ＲＩ），对９种糖和五种糖醇进行了分离检测；另以对甲氧基苯胺和苯甲酰氯为衍生试剂，分别对糖和糖醇进行柱前衍生，使其接上紫外吸收基团，然后用紫外检测器（ＵＶ）进行检测。在０．５￣１２ｇ／Ｌ浓度范围内，对糖和糖醇浓度与其衍生物峰面积进行线性回归分析，其相关系数在０．９８４￣０．９９８之间；ＵＶ的检测灵敏度比ＲＩ的要高约１０００倍。
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| 关 键 词： | 糖 糖醇 高效液相色谱 |
Analysis of Sugars and Sugar Alcohols by High Performance Liquid Chromatography |
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| Xu Xiuzhu,Xu Genliang.Analysis of Sugars and Sugar Alcohols by High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,1999,27(5):547-550. |
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| Authors: | Xu Xiuzhu Xu Genliang |
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| Abstract: | Nine sugars and five sugar alcohols were analysed by HPLC with a differenctial refractometer (RI)detector. p-Methoxyaniline and benzoyl chloride were used to the pre-column derivatization of sugar and sugar alcohol, in which a ultraviolet absorption organic group was added, Then the derivatives were detected sensitively by an UV monitor sensitively. Its sensitivity was 1000 times higher than that of the RI detector. The linear regression equations between the concentrations of sugar and sugar alcohol and their peak areas of the derivatives were established in the range of 0.5 -12 rng/L. The correlation coefficients were between 0.984-0.998. |
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| Keywords: | Sugar sugar alcohol high performance liquid chromatographv |
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