| 荧光光谱法研究喹诺酮抗菌素与过氧化氢酶的相互作用 |
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| 引用本文： | 易平贵,商志才,俞庆森.荧光光谱法研究喹诺酮抗菌素与过氧化氢酶的相互作用[J].分析化学,2001,29(6):646-648. |
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| 作者姓名： | 易平贵 商志才 俞庆森 |
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| 作者单位： | 1. 湘潭工学院化学工程系,
2. 浙江大学化学系, |
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| 基金项目： | 国家自然科学科学基金重点项目资助课题（批准号：29633020） |
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| 摘 要： | 应用荧光光谱法研究了水溶液中喹诺酮抗菌素氧氟沙星、环丙沙星与过氧化氢酶分子间的结合反应。结果表明：药物对过氧化氢酶的内源荧光有较强的猝灭作用，形成复合物所产生的静态猝灭是引起过氧化氢酶荧光猝灭的主要原因。进一步依据荧光猝灭结果确定了药物－酶复合物的形成常数和结合位点数。
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| 关 键 词： | 喹诺酮 过氧化氢酶 结合反应 荧光光谱 |
| 修稿时间： | 2000年7月21日 |
Interaction Between Quinolone Antibacterial Agents and Catalase by Fluorescence Spectroscopy |
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| Yi Pinggui,Shang Zhicai,Yu Qingsen.Interaction Between Quinolone Antibacterial Agents and Catalase by Fluorescence Spectroscopy[J].Chinese Journal of Analytical Chemistry,2001,29(6):646-648. |
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| Authors: | Yi Pinggui Shang Zhicai Yu Qingsen |
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| Abstract: | The binding characteristics of quinolone antibacterial agent, ofloxacin (OFLX) and ciprofloxacin(CPFX) with catalase(CAT) were studied by fluorescence spectroscopy in aqueous solution. It was showed that both OFLX and CPFX had a strong ability to quench the CAT intrinsic fluorescence through a static quenching procedure. The formation constant K and the number of binding site n were further calculated according to the fluorescence quenching results. |
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| Keywords: | Quinolone catalase interaction fluorescence spectroscopy |
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