| 高效液相色谱-电喷雾质谱联用法测定人参和西洋参的皂苷类成分 |
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| 引用本文： | 李丽,刘春明,吴巍,越皓,刘志强,刘淑莹,田成.高效液相色谱-电喷雾质谱联用法测定人参和西洋参的皂苷类成分[J].分析化学,2005,33(8):1087-1090. |
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| 作者姓名： | 李丽 刘春明 吴巍 越皓 刘志强 刘淑莹 田成 |
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| 作者单位： | 中国科学院长春应用化学研究所,长春,130022;长春师范学院化学系,长春,130032;长春师范学院化学系,长春,130032;中国科学院长春应用化学研究所,长春,130022;满洲里检验检疫技术中心,满洲里,021400 |
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| 基金项目： | 国家自然科学基金;中国科学院知识创新工程项目;吉林省科技发展计划 |
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| 摘 要： | 利用高效液相色谱（LC—ELSD）与电喷雾质谱（ESI—MS）联用技术对人参和西洋参中的人参皂苷类成分进行了比较研究。通过液相色谱与质谱联用技术获得了相应化合物的分子量信息；利用质谱的源内CID技术获得了相应化合物的结构信息。根据人参皂苷Rf和拟人参皂苷F11两种同分异构体在质谱中的源内CID裂解规律的不同，建立了人参和西洋参药材的LC-ESI-MS简便、准确的鉴别方法。
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| 关 键 词： | 人参 西洋参 人参皂苷Rf 拟人参皂苷F11 高效液相色谱-电喷雾质谱联用 源内CID质谱技术 |
| 收稿时间： | 05 31 2004 12:00AM |
| 修稿时间： | 2004-05-31 |
Analysis of Saponins in Extract of Panax Ginseng and Panax Quinquofolium by Liquid Chromatography-Electrospray Ionization Mass Spectrometry |
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| Li Li,LIU Chunming,Wu Wei,Yue Hao,Liu Zhiqiang,Liu Shuying,Tian Cheng.Analysis of Saponins in Extract of Panax Ginseng and Panax Quinquofolium by Liquid Chromatography-Electrospray Ionization Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2005,33(8):1087-1090. |
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| Authors: | Li Li LIU Chunming Wu Wei Yue Hao Liu Zhiqiang Liu Shuying Tian Cheng |
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| Abstract: | A Liquid chromatography-electrospray ionizaiton mass spectrometry (LC-ESI-MS) method was (developed) for the identification and differentiation of ginsenosides in crude extracts from Panax ginseng and Panax quinquofolium L. The ~- ions of ginsenosides were observed in the negative mode, from which the molecular weights were obtained. The collision induced dissociation (CID) data of the ~- ions (provide) detailed structural information of the ginsenosides. By performing CID experiments for the peaks (3(ginsenoside Rf)) and 8(Pseudoginsenoside F11), these two ginsenosides had different CID behavior, and therefore the (assignment) of the structure for these two isomers was straightforward. The experimental results (indicate) that P. ginseng and P. quinquofolium can be differentiated rapidly and easily by the LC-ESI-MS (experiment). |
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| Keywords: | P ginseng P quinquofolium L ginsenoside Rf pseudoginsenoside F11 liquid chromatogra phy-electrospray ionization mass spectrometry collision induced dissociation |
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