| 基于母离子排除的串联质谱数据采集方法的研究 |
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| 引用本文： | 米薇,王晶,应万涛,贾伟,蔡耘,钱小红.基于母离子排除的串联质谱数据采集方法的研究[J].分析化学,2010,38(2). |
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| 作者姓名： | 米薇 王晶 应万涛 贾伟 蔡耘 钱小红 |
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| 作者单位： | 1. 中国计量科学研究院生物能源环境所,北京,100013
2. 北京蛋白质组研究中心,蛋白质组学国家重点实验室,北京放射与辐射医学研究所,北京,102206 |
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| 基金项目： | 国家高技术研究发展计划(No.2006AA02A308);;国家重点基础研究发展计划(No.2006CB910801);;国家自然科学基金(No.20735005);;国家科技重大专项(No.2008ZX08012-003);;国家科技支撑计划(No.2006BAK04A02)资助 |
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| 摘 要： | 生物样本中的蛋白质复杂程度很高,尚无一种分析方法能够全面分析复杂生物样本中的所有蛋白质。实验证明:重复的质谱分析能增加蛋白质的鉴定数量,但鉴定到的蛋白质冗余度很高,高丰度肽段被反复检出。为了降低鉴定冗余度,提高鉴定效率,对线性离子阱-傅立叶变换离子回旋共振质谱仪的串联质谱采集方法进行了研究,建立了基于母离子排除的串联质谱采集方法。此方法能极大降低二级质谱采集的冗余度,提高蛋白质的鉴定效率。
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| 关 键 词： | 液相色谱-串联质谱 母离子排除列表 串联质谱数据采集 |
Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions |
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| MI Wei,WANG Jing,YING Wan-Tao,JIA Wei,CAI Yun,QIAN Xiao-Hong.Tandem Mass Spectrometry Acquisition Strategy Based on Exclusion of Precursor Ions[J].Chinese Journal of Analytical Chemistry,2010,38(2). |
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| Authors: | MI Wei WANG Jing YING Wan-Tao JIA Wei CAI Yun QIAN Xiao-Hong |
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| Institution: | National Institute of Metrology;Division of Biological;Energy and Environment Measurement;Beijing 100013;State Key Laboratory of Proteomics;Beijing Proteome Research Center;Beijing Institute of Radiation Medicine;Beijing 102206 |
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| Abstract: | Due to the complexity of proteome samples,comprehensive analysis to characterize all proteins was still not possible with present methodologies.It has been shown that replicate runs could increase the number of identified proteins.However,the redundancy of protein identifications was high.High-abundant peptides tended to be analyzed repeatedly in different runs.To reduce the redundancy and improve the efficiency of identification,we studied the MS/MS acquisition method of linear ion trap Fourier transform i... |
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| Keywords: | Liquid chromatography-electrospray ionization mass spectrometry/mass spectrometry Precursor ion exclusion list Tandem mass spectrometry acquisition |
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