在纤维素衍生物类手性柱上分离托品酸对映体

以乙醇-水为流动相,用纤维素-三(苯甲酯)(CTB)作为手性固定相对外消旋体托体品酸进行了高效液相色谱手性分离,考察了不同比例的乙醇-水流动相,不同流速以及用不同醇类酯对托品酸酯在手性色谱柱上的色谱行为,实验表明,流动相中水的比例,流速以及酯化所用醇类均对托品酸酯衍生物分离有很大影响,以动相为乙醇-水(95:5)流速为0.1mL/min的色谱体系,使托品酸乙酯在CTB柱上得到基线分离,对托品酸乙酯在CTB手性固定相上反相手性识别机理进行了讨论.

Enantiomeric Separation of Tropine Acid on High Performance Liquid Chromatography with Cellulose Derivatives as Chiral Column

Reverse-phase chromatography was employed for chiral separationof tropine acid enantiomers on the cellulose tribenzoate (CTB) chiral stationary phases coated on a small-particle spherical silica support in an eluant system consisting of a mixture of alcohol and water. The influence of water proportion in mobile phase, flow rate of chromatography, derivatives of various kinds of alcohol were systematically examined. The result showed that the mobile phase strength, flow rate of chromatography and the structure of chiral compounds were dominant factors to retention and resolution of the analytes. Meanwhile, the steric effects of alcohol seemed to be a certain factor. We observed that a good separation of enantiomers of tropine acid ethyl ester derivatives could be obtained under the conditions of chromatography, which was mobile phase： alcohol-water (95∶5); flow rate: 0.1 mL/min; stationary phase: cellulose tribenzoate.