| 高产花生四烯酸产生菌干菌体中脂肪酸成分的气相色谱-质谱分析 |
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| 引用本文： | 王相勤,姚建铭,袁成凌,余增亮.高产花生四烯酸产生菌干菌体中脂肪酸成分的气相色谱-质谱分析[J].分析化学,2001,29(3):287-289. |
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| 作者姓名： | 王相勤 姚建铭 袁成凌 余增亮 |
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| 作者单位： | 中国科学院等离子体物理研究所离子束生物工程实验室, |
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| 基金项目： | 安徽省科技攻关项目，85-722-22-01， |
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| 摘 要： | 将高产生花生四烯酸（AA）脂肪酸产生菌的干菌体经三氟化硼－甲醇方法甲酯化后，用毛细管气相色谱和质谱进行分析。结果表明干菌体中含有饱和不饱和脂肪酸，其中不饱和脂肪数中以花生四烯酸的含量（70．20％）最高，另外还含有少量的亚油酸和γ-亚麻酸等不饱和脂肪酸，同时给出了各个脂肪酸组分的相对含量。
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| 关 键 词： | 脂肪酸 气相色谱－质谱联用 花生四烯酸产生菌 干菌体 分析 发酵法生产 |
The Analysis of Fatty Acids of Dry-Thallus High-Yield ofArachidonic Acid Producing Strain byGas Chromatography and Mass Spectrometry |
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| Wang Xiangqin,Yao Jianming,Yuan Chengling,Yu Zengliang.The Analysis of Fatty Acids of Dry-Thallus High-Yield ofArachidonic Acid Producing Strain byGas Chromatography and Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2001,29(3):287-289. |
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| Authors: | Wang Xiangqin Yao Jianming Yuan Chengling Yu Zengliang |
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| Abstract: | A high-yield arachidonic acid producing strain (mortierella alpina) was selected with ion implantation. After treating the dry-thallus of high-yield arachidonic acid-producing strain with BF3-CH3CH solution, the fatty acid in dry-thallus of arachidonic acid producing strain were analyzed by gas chromatography and mass spectrometry. The result showed that there were saturated and unsaturated fatty acids in the dry-thallus. The relative content of arachidonic acid is 70.2% in the all fatty acids. In addition, there were oleic acid, linoleic acid and γ-linolenic acid in the dry-thallus. |
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| Keywords: | Arachidonic acid high-yield strain fatty acid gas chromatography mass spectrometry |
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