| 限制性内切酶指纹-高效毛细管电泳激光诱导荧光法测定原核/真核质粒 |
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| 引用本文： | 王荣,贾正平,阮金秀,谢华,陈巧云,贾海,张强,徐娟,敖燕.限制性内切酶指纹-高效毛细管电泳激光诱导荧光法测定原核/真核质粒[J].分析化学,2007,35(8):1137-1141. |
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| 作者姓名： | 王荣 贾正平 阮金秀 谢华 陈巧云 贾海 张强 徐娟 敖燕 |
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| 作者单位： | 1. 兰州军区兰州总医院临床药理基地,兰州,730050
2. 军事医学科学院毒物药物研究所,北京,100850 |
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| 基金项目： | 甘肃省自然科学基金资助项目(NoZS031-A25-70-E)，中国博士后基金资助项目(No2005037576)，全军“十一五”科研课题面 |
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| 摘 要： | 采用线性聚丙烯酰胺修饰石英毛细管的高效毛细管电泳无胶筛分技术,对原核/真核质粒用不同限制性内切酶酶切,运用限制性内切酶指纹-高效毛细管电泳激光诱导荧光(REF-HPCE-LIF)检测法同时对内切酶酶切后多个和较长DNA片段进行了检测,电泳缓冲液为1×TBE(pH8.3),阴极电压进样(10kV,5s),分离电压13kV,25℃,激光诱导荧光检测器检测(λex=520nm)。结果表明,所建立的REF-HPCE-LIF方法可对原核/真核酶切后多个和较长DNA片段进行检测,获得了满意的限制性内切酶指纹图谱,能够检测片段大小相差不超过10bp。所建立的方法较琼脂糖电泳分辨率高,可应用在检测多个和较长DNA片段的突变,在诊断肿瘤方面有一定应用前景。
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| 关 键 词： | 高效毛细管电泳 限制性内切酶 原核/真核质粒 激光诱导荧光 |
| 修稿时间： | 2006-11-02 |
Determination of the Plasmid of Protokaryon and Enkaryon by the Restriction Endonuclease Fingerprinting-Capillary Electrophoresis with Laser Induced Fluorescence Detection |
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| Wang Rong,Jia Zheng-Ping,Ruan Jin-Xiu,Xie Hua,Chen Qiao-Yun,Jia Hai,Zhang Qiang,Xu Juan,Ao Yan.Determination of the Plasmid of Protokaryon and Enkaryon by the Restriction Endonuclease Fingerprinting-Capillary Electrophoresis with Laser Induced Fluorescence Detection[J].Chinese Journal of Analytical Chemistry,2007,35(8):1137-1141. |
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| Authors: | Wang Rong Jia Zheng-Ping Ruan Jin-Xiu Xie Hua Chen Qiao-Yun Jia Hai Zhang Qiang Xu Juan Ao Yan |
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| Institution: | Department of Pharmacy, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050;Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850 |
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| Abstract: | |
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| Keywords: | Capillary electrophoresis restriction endonuclease fingerprinting plasmid of protokaryon and enkaryon laser induced fluorescence detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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