| 壳聚糖支载菁染料荧光探针及荧光特性 |
| |
| 引用本文： | 刘志军,费学宁,谷迎春.壳聚糖支载菁染料荧光探针及荧光特性[J].影像科学与光化学,2009,27(4):307-313. |
| |
| 作者姓名： | 刘志军 费学宁 谷迎春 |
| |
| 作者单位： | 天津城市建设学院材料科学与工程系,天津,300384 |
| |
| 基金项目： | 国家自然科学基金，天津市应用基础及前沿技术研究计划重点项目 |
| |
| 摘 要： | 壳聚糖支载有机荧光染料可在生理条件下改变其荧光特性,本文采用壳聚糖(CTS)支载有机荧光染料Cy3,利用红外光谱和热分析对产物进行了表征,研究了壳聚糖支载的荧光染料紫外吸收光谱和荧光光谱特性,结果表明,壳聚糖支载的荧光染料Cy3的紫外吸收特征峰的波长无明显变化,荧光强度明显增强,支载后的荧光染料光稳定性良好,初步细胞标记实验显示,壳聚糖支载后的Cy3染料对脑胶质瘤细胞具有良好的亲和性,并能穿透细胞膜且发出明显荧光。
|
| 关 键 词： | Cy3 壳聚糖支栽 荧光特征 紫外吸收 细胞标记 |
Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes |
| |
| LIU Zhi-jun,FEI Xue-ning,GU Ying-chun.Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes[J].Imaging Science and Photochemistry,2009,27(4):307-313. |
| |
| Authors: | LIU Zhi-jun FEI Xue-ning GU Ying-chun |
| |
| Abstract: | Organic fluorescent Dyes can be supported by Chitosan and the fluorescent Characteristics in physiological environment can be changed.In this paper,unsymmetrical water-soluble cyanine dye(Cy3) was supported by Chitosan(CTS),and the product(Cy3-CTS) was characterized by IR and DTA.The fluorescence Characteristics and ultraviolet absorbance were studied.The results showed that the characteristic peak of UV absorption spectrum of Cy3-CTS shifted little compared to that of Cy3,but the fluorescence intensity enhanced obviously with good photostability.Primary experimental study on labeled cells indicated that Cy3-CTS can pass through the cell membrane with good affinity to glioma cell and show strong fluorescence. |
| |
| Keywords: | Cy3 |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
