| 离子交换法从发酵液中提取L-异亮氨酸 |
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| 引用本文： | 刘清华,张伟国,钱和.离子交换法从发酵液中提取L-异亮氨酸[J].离子交换与吸附,2003,19(5):457-462. |
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| 作者姓名： | 刘清华 张伟国 钱和 |
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| 作者单位： | 1. 江南大学食品学院,无锡,214036
2. 江南大学生物工程学院,无锡,214036 |
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| 摘 要： | 采用天然高分子絮凝剂壳聚糖对L-异亮氨酸发酵液进行预处理，在发酵液pH=2，壳聚糖用量30mg／L的条件下可取得较好的絮凝效果．并通过静态吸附实验，考察了pH和L—异亮氨酸浓度对平衡吸附量的影响．最后确定了732″离子交换树脂提取L-异亮氨酸的最佳工艺条件：上柱发酵液pH=2，上样速度0．6BV／h，洗脱液为0.5mol／L的NH4Cl．流出液经脱色、浓缩结晶后得L-异亮氨酸成品，总提取率为55％．
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| 关 键 词： | 离子交换法 发酵液 提取 L-异亮氨酸 工艺条件 预处理 壳聚糖 絮凝剂 |
| 文章编号： | 1001-5493(2003)05-0457-06 |
| 修稿时间： | 2003年4月2日 |
RECOVERY OF L-ISOLEUCINE FROM FERMENTATION BROTH VIA ION EXCHANGE |
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| LIU Qinghua ZHANG Weiguo QIAN He School of Food Science & Technology,Southern Yangtze University,Wuxi ,China School of Biotechnology,Southern Yangtze University,Wuxi ,China.RECOVERY OF L-ISOLEUCINE FROM FERMENTATION BROTH VIA ION EXCHANGE[J].Ion Exchange and Adsorption,2003,19(5):457-462. |
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| Authors: | LIU Qinghua ZHANG Weiguo QIAN He School of Food Science & Technology Southern Yangtze University Wuxi China School of Biotechnology Southern Yangtze University Wuxi China |
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| Institution: | LIU Qinghua1 ZHANG Weiguo2 QIAN He1 1 School of Food Science & Technology,Southern Yangtze University,Wuxi 214036,China 2 School of Biotechnology,Southern Yangtze University,Wuxi 214036,China |
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| Abstract: | Pretreatment of L-isoleucine fermentation broth by using chitosan. Excellent coagulation result was achieved under the following condition: pH2.0, chitosan dosage 30mg/L. Effects of factors such as pH and concentration of L-isoleucine on absorption capacity have been investigated by static state absorption experiment. The optimal operation conditions of extracting L-isoleucine were as following: fermentation broth pH2, sample flow-rate 0.6BV/h, 0.5mol/L NH4Cl as eluant, then effluent was decolored, concentrated and crystallized. The total recovery ratio is 55%. |
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| Keywords: | L-Isoleucine Ion exchange Recovery Process conditions |
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