| 双功能交联分子对生物大分子DNA磷酸骨架的修饰 |
| |
| 引用本文： | 马世奎,王莹,王影,宓怀风.双功能交联分子对生物大分子DNA磷酸骨架的修饰[J].离子交换与吸附,2011,27(6). |
| |
| 作者姓名： | 马世奎 王莹 王影 宓怀风 |
| |
| 作者单位： | 功能高分子材料教育部重点实验室,南开大学高分子化学研究所,天津300071 |
| |
| 基金项目： | 国家自然科学基金(21004034) |
| |
| 摘 要： | 利用DNA分子在水溶液中解离出的阴离子通过亲核取代反应亲核进攻重氮基乙酸叔丁酯的易离去基团重氮基来完成对DNA磷酸骨架的修饰。我们用NMR(31P)与琼脂糖凝胶电泳对结果进行表征,结果表明,DNA磷酸骨架上部分磷原子的羟基被重氮基乙酸叔丁酯修饰。
|
| 关 键 词： | 磷酸骨架 DNA 重氮基乙酸叔丁酯 修饰 |
THE MODIFICATION OF DNA WITH BIFUNCTIONAL CROSSLINKER TERT-BUTYL DIAZOACETATE THROUGH CAGING STRATEGY |
| |
| MA Shikui,WANG Ying,WANG Ying,MI Huaifeng.THE MODIFICATION OF DNA WITH BIFUNCTIONAL CROSSLINKER TERT-BUTYL DIAZOACETATE THROUGH CAGING STRATEGY[J].Ion Exchange and Adsorption,2011,27(6). |
| |
| Authors: | MA Shikui WANG Ying WANG Ying MI Huaifeng |
| |
| Institution: | MA Shikui WANG Ying WANG Ying MI Huaifeng Key Laboratory of Functional Polymer Materials,Ministry of Education of China,Institute of Polymer Chemistry,Nankai University,Tianjin 300071,China |
| |
| Abstract: | The modified deoxyribonucleic acid(DNA) bearing tert-butyl acetate moiety was synthesized by the reaction of the anion of phosphate with tert-butyl diazoacetate in aqueous solution,and its structure was determined by NMR(31P) and agarose gel electrophoresis.The results showed that the tert-butyl acetate group was introduced to DNA backbone. |
| |
| Keywords: | Phosphate backbone DNA Tert-butyl diazoacetate Modification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
