| 球状苯基纤维素疏水性吸附树脂分离生物活性蛋白质的研究 |
| |
| 引用本文： | 朱云,朱建华,肖锦.球状苯基纤维素疏水性吸附树脂分离生物活性蛋白质的研究[J].离子交换与吸附,2000,16(6):514-520. |
| |
| 作者姓名： | 朱云 朱建华 肖锦 |
| |
| 作者单位： | 1. 华南理工大学造纸与环境工程学院,广州 510640
2. 华南理工大学造纸与环境工程学院应化系,广州 510640 |
| |
| 摘 要： | 本文考察了球状苯基纤维素疏水性吸附树脂对三种生物活性蛋白质：精制木瓜酶、中性蛋白酶和菠萝蛋白的吸附分离情况。研究表明，该树脂对菠蛋白酶的吸附分离情况较好，一步富集倍数和活性回收率高，活性回收率达１２８％，浓缩倍数达４．４，进一步盐析干燥可制得固体晶状菠萝酶。
|
| 关 键 词： | 疏水性吸附树脂 球状苯基纤维素 生物活性蛋白质 |
| 文章编号： | 1001-5493(2000)06-0514-07 |
| 修稿时间： | 2000年2月5日 |
ISOLATION OF BIO-ACTIVE PROTEINS BY SPHERICAL PHENYL-CELLULOSE HYDROPHOBIC ADSORPTIVE RESIN |
| |
| ZHU Yun,ZHU Jianhua,XIAO Jin,Paper Making and Environmental Engineering Institute,Applied Chemical.ISOLATION OF BIO-ACTIVE PROTEINS BY SPHERICAL PHENYL-CELLULOSE HYDROPHOBIC ADSORPTIVE RESIN[J].Ion Exchange and Adsorption,2000,16(6):514-520. |
| |
| Authors: | ZHU Yun ZHU Jianhua XIAO Jin Paper Making and Environmental Engineering Institute Applied Chemical |
| |
| Institution: | ZHU Yun1 ZHU Jianhua2 XIAO Jin1 1 Paper Making and Environmental Engineering Institute 2 Applied Chemical Department,South China University of Technology,Guangzhou,510640 |
| |
| Abstract: | The adsorption and isolation of three sorts of bio-active proteins: papain, neutral albumen enzyme, and bramelain are reported. The results show that: the bio-active bramelain could be successfully separated from crude stem bramelain juice by spherical phenyl-cellulose hydrophobic adsorptive resin, and 128% of total activity with 4.4 fold enrichment was obtained at only one step; moreover after ulteriorly treatment and drying the needle-shaped solid bramelain was gained. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
