| 聚离子亚基化合物结构影响外源基因转染效率 |
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| 引用本文： | 史国利,游环宇.聚离子亚基化合物结构影响外源基因转染效率[J].离子交换与吸附,1999,15(1):77-80. |
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| 作者姓名： | 史国利 游环宇 |
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| 作者单位： | 南开大学分子生物学研究所!天津300071(史国利，游环宇，陈德风)，南开大学高分子化学研究所!天津300071(印寿根) |
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| 摘 要： | 合成了带正电荷密度不同的聚离子亚基化合物，探讨了聚合物结构对外源基因转染真核细胞的影响规律。从实验结果看，聚离子亚基化合物结构影响外源基因转染效率，但其影响程度与靶细胞的种类相关，对ＨｅＬａ细胞，ＮＩＨ３Ｔ３细胞，聚离子亚基化合物的正电荷密度加大，其促基因转移功能加强；而对ＰＡ３１７包装细胞，结果相反。
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| 关 键 词： | 聚离子亚基 转染 基因表达 基因转移 Polybrene |
THE STRUCTURES OF THE IONENE POLYMERS AFFECTED THE EFFICIENCY OF EXOGENOUS TRANSIENT TRANSFECTION |
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| Shi Guoli, You Huanyu, Yin Shougen, Chen Defeng.THE STRUCTURES OF THE IONENE POLYMERS AFFECTED THE EFFICIENCY OF EXOGENOUS TRANSIENT TRANSFECTION[J].Ion Exchange and Adsorption,1999,15(1):77-80. |
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| Authors: | Shi Guoli You Huanyu Yin Shougen Chen Defeng |
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| Abstract: | Using synthetic and different positive charge density ionene polymers and the effect law of their structures on transient expression of exogenous genes in eukaryocytes were determined.The results showed the structures of the ionene polymers affected the efficiency of exogenous transient transformation, the levels of effect were associated with the kinds of target cells. In HeLa and NIH3T3 cells, with the increasing of positive charge density of ionene polymers, the function of their promoting gene transfer enhanced, but in PA317 package cells, the results were opposite. The results also indicated that the extra high or low concentrations of ionene polymers reduced the efficiency of exogenous gene transfer. |
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| Keywords: | Ionene polymers Gene transfer Transient expression |
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