| 魔芋葡甘聚糖DEAE阴离子交换介质的制备及表征 |
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| 引用本文： | 葛佳丽,王佳兴,马光辉,王芳,邓利.魔芋葡甘聚糖DEAE阴离子交换介质的制备及表征[J].离子交换与吸附,2007,23(4):289-296. |
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| 作者姓名： | 葛佳丽 王佳兴 马光辉 王芳 邓利 |
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| 作者单位： | 1. 北京化工大学生命科学与技术学院,北京,100029
2. 中国科学院过程工程研究所国家生化重点实验室,北京,100080 |
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| 摘 要： | 以交联的魔芋葡甘聚糖微球(KGM)为基质,制备了DEAE型的阴离子交换层析介质,考察了反应物浓度、温度、时间等反应因素对魔芋葡甘聚糖层析介质离子交换容量的影响,确定最优反应参数.结果表明,层析介质的离子交换容量达到3.42mmol/g,蛋白吸附容量高达126.84mg/mL,蛋白洗脱率为90.42%.
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| 关 键 词： | 魔芋葡甘聚糖 阴离子交换介质 离子交换容量 |
| 文章编号： | 1001-5493(2007)04-0289-08 |
| 修稿时间： | 2006-11-23 |
PREPARATION AND CHARACTERIZATION OF ANION EXCHANGER DEAE-KONJAC GLUCOMANNAN MICROSPHERES |
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| GE Jiali,MA Guanghui,WANG Jiaxing,WANG Fang,DENG Li.PREPARATION AND CHARACTERIZATION OF ANION EXCHANGER DEAE-KONJAC GLUCOMANNAN MICROSPHERES[J].Ion Exchange and Adsorption,2007,23(4):289-296. |
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| Authors: | GE Jiali MA Guanghui WANG Jiaxing WANG Fang DENG Li |
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| Institution: | 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.2. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. |
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| Abstract: | DEAE anion exchangers were prepared by crossing diethylaminoethyl (DEAE) onto konjac glucommannan gel. Effects of reactants concentration, reaction temperature, reaction time and other reaction factors on exchange capacity of konjac glucommannan gel were studied and optimized reaction parameters were obtained. Under the optimum reaction condition, the exchange capacity of the prepared gel was 3.42mmol/g, the adsorption capacities for protein was 126.84mg/mL, and the desorption reached 90.42%. |
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| Keywords: | DEAE |
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