高效液相色谱法测定野葱中黄酮类化合物

建立了测定野葱中芦丁、黄酮醇类化合物的高效液相色谱方法。80%乙醇超声提取,高效液相色谱分析野葱中芦丁和黄酮醇类化合物的含量。芦丁检测条件:V(甲醇)∶V(0.2%磷酸水)=45∶55,检测波长:360nm;槲皮素、山奈酚、异鼠李素检测条件:V(甲醇)∶V(0.2%磷酸水)=40∶60,检测波长:360 nm。结果表明,野葱中芦丁含量0.22%,槲皮素、山奈酚、异鼠李素含量依次为0.42%、0、0.23%,总黄酮醇类化合物含量为1.63%。

Determination of flavonoid compounds in Allium Chrysanthum by high performance liquid chromatography

A method for determination of rutin and flavonols in Allium Chrysanthum by high performance liquid chromatography was established.Allium Chrysanthum powder was extracted with ethanol of 80%by ultrasonic technique.Rutin and flavonols(quercetin,kaempferol and isoharmnten)were detected by high performance liquid chromatography.The detecting condition of rutin was that the volume ratio of methanol and 0.2%phosphoric acid was 45:55 and detection wavelength was 360nm.The detection wavelength of flavonols was the same as rutin,but the volume ratio of methanol and 0.2%phosphoric acid was 40:60.The results showed that the rutin content was 0.22%,while the quercetin,kaempferol and isorhamnetin content reached 0.42%,0,0.23%respectively,and the total flavonols content was 1.63%in Allium Chrysanthum.