| 曙红B及变色酸与核酸作用的光谱学性质研究 |
| |
| 引用本文： | 王海燕,栾尼娜,宋玉民.曙红B及变色酸与核酸作用的光谱学性质研究[J].化学研究与应用,2009,21(4). |
| |
| 作者姓名： | 王海燕 栾尼娜 宋玉民 |
| |
| 作者单位： | 西北师范大学化学化工学院,甘肃,兰州,730070 |
| |
| 基金项目： | 甘肃省自然科学基金，甘肃省高分子材料重点实验室项目 |
| |
| 摘 要： | 利用荧光光谱法、紫外-可见吸收光谱法、圆二色谱法、凝胶电泳法对曙红B及变色酸与核酸的作用机理进行了探讨. 当ct-DNA浓度增加时,曙红B和变色酸的荧光光谱呈荧光增强趋势;紫外-可见光谱呈下降趋势;并使ct-DNA圆二色谱正负峰吸收强度呈现一定的降低;琼脂糖凝胶电泳实验中,二者在泳道中与DNA作用发出的荧光在紫外灯下皆清晰可见. 并将所得结果与溴化乙锭-DNA体系性质进行对比,为寻找高效低毒的DNA分子荧光探针提供了实验基础.
|
| 关 键 词： | 曙红B 变色酸 溴化乙锭 核酸 光谱学性质 |
The interactions of eosin B and chromotropic acid with DNA |
| |
| WANG Hai-yan,LUAN Ni-na,SONG Yu-min.The interactions of eosin B and chromotropic acid with DNA[J].Chemical Research and Application,2009,21(4). |
| |
| Authors: | WANG Hai-yan LUAN Ni-na SONG Yu-min |
| |
| Institution: | College of Chemistry and Chemical Engineering;Northwest Normal University;Lanzhou 730070;China |
| |
| Abstract: | The interactions between Eosin B and DNA,chromotropic acid and DNA are studied by fluorescence,UV-absorption,circular dichroism spectral and agarose gel electrophoresis methods.When DNA exists,the fluorescence intensity of Eosin B and chromotropic acid put up a direction of increasing.The absorbance of UV spectra both present a hypochromic effect when DNA concentration rise.The CD spectra of DNA has a great degressive both in negative and positive spectra with Eosin B and chromotropic acid added.In the agar... |
| |
| Keywords: | eosin B Chromotropic acid ethidium bromide DNA spectral character |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
