| 提高漆酶稳定性的化学修饰方法的研究 |
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| 引用本文： | 熊亚红,高敬忠,郑坚鹏.提高漆酶稳定性的化学修饰方法的研究[J].化学研究与应用,2011,23(8):985-990. |
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| 作者姓名： | 熊亚红 高敬忠 郑坚鹏 |
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| 作者单位： | 华南农业大学理学院生物材料研究所,广东 广州,510642 |
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| 基金项目： | 华南农业大学“211工程”重点学科建设项目(2009B010100001)资助; 国家自然科学基金(青年科学基金项目)(20906034B0604)资助; 广东省科技计划项目(2008B080701011)资助; 国家林业局948项目(2011-4-75)资助 |
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| 摘 要： | 天然漆酶在其应用条件下容易失活,因此如何提高漆酶的稳定性显得尤为重要和紧迫.以目前广泛应用的商品化漆酶DeniLite ⅡS为研究对象,采用邻苯二甲酸酐(PA)、丁二酸酐(SA)及马来酸酐(MA)对漆酶进行化学修饰,根据修饰酶的酶活和热稳定性的变化确定SA为漆酶的最佳修饰剂.采用L9(34)正交设计表研究了磷酸盐缓冲液...
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| 关 键 词： | 漆酶 化学修饰 酶活力 稳定性 |
Study on the chemical modification of Laccase with stability enhancement |
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| XIONG Ya-hong,GAO Jing-zhong,ZHEN Jian-peng.Study on the chemical modification of Laccase with stability enhancement[J].Chemical Research and Application,2011,23(8):985-990. |
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| Authors: | XIONG Ya-hong GAO Jing-zhong ZHEN Jian-peng |
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| Institution: | XIONG Ya-hong,GAO Jing-zhong,ZHEN Jian-peng(Institute of Biomaterial,College of Science,South China Agricultural University,guangzhou 510642,China) |
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| Abstract: | Native laccase was applied widely in industry.It was becoming very important and urgent because native laccase was amenable to activity loss under its application conditions.In this paper,a commercial native laccase named DeniLite IIS with wide application was researched.This laccase was modified with different anhydrides,such as phthalic anhydride(PA),succinic anhydride(SA)and maleic anhydride(MA).SA was confirmed as a best modification reagent for laccase according to enzymatic activity and stability enha... |
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| Keywords: | Laccase chemical modification enzymatic activity stability |
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