| 荧光反猝灭法测定药剂中卡托普利 |
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| 引用本文： | 戴兴德,石欢,陈盈盈,张小林.荧光反猝灭法测定药剂中卡托普利[J].化学研究与应用,2021,33(1):201-204. |
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| 作者姓名： | 戴兴德 石欢 陈盈盈 张小林 |
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| 作者单位： | 甘肃医学院药学系,甘肃 平凉 744000;甘肃医学院药学系,甘肃 平凉 744000;甘肃医学院药学系,甘肃 平凉 744000;甘肃医学院药学系,甘肃 平凉 744000 |
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| 基金项目： | 甘肃省高等学校创新能力提升项目(2020A-163)资助。 |
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| 摘 要： | 建立荧光反猝灭法测定卡托普利(CAT)的体系。碘(I-3)与罗丹明B(RhB)缔合作用使罗丹明B荧光信号强度减弱直至荧光猝灭,而卡托普利可将I-3还原为I-,使体系荧光信号再现。在2~20μmol·L-1范围内荧光强度再现值ΔF与卡托普利浓度有线性关系:ΔF=-8.438+10.774 c(r=0.9986),检出限为3.34×10-9 mol·L-1。方法用于药剂卡托普利含量测定,测定值与标示量一致,加标回收率在95.2%~102.3%之间。
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| 关 键 词： | 罗丹明B 碘 卡托普利 荧光反猝灭法 |
Determination of captopril in pharmaceutical based on fluorescence inverse quenching |
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| DAI Xing-de,SHI Huan,CHEN Ying-ying,ZHANG Xiao-lin.Determination of captopril in pharmaceutical based on fluorescence inverse quenching[J].Chemical Research and Application,2021,33(1):201-204. |
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| Authors: | DAI Xing-de SHI Huan CHEN Ying-ying ZHANG Xiao-lin |
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| Institution: | (Department of pharmacy,Gansu Medical College,Pingliang 744000,China) |
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| Abstract: | A new fluorescence system was developed for the determination of captopril(CAT)in pharmaceutical.When the fluorescence signal intensity of Rhodamine B(RhB)was weakened or even disappeared by Iodine(I-3)solution,Captopril could reduce I-3 to I-,which made the system fluorescent signal reappear.The recovery value of fluorescence intensityΔF had a linear relationship with the concentration of captopril in the range of2~20μmol·L-1,The linear regression equation wasΔF=-8.438+10.774 c(r=0.9986),The detection limit was 3.34×10-9 mol·L-1.Captopril tablets were determined by this method,the measured values were consistent with the marked quantity,and the recovery rate was between 95.2%~102.3%. |
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| Keywords: | rhodamine B iodine captopril fluorescence inverse quenching |
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