首页 | 本学科首页   官方微博 | 高级检索  
     检索      

麻风树种子中毒蛋白的提取分离
引用本文:梁渠,闫书一,李瑜,陈康生,何文琼,何丽丽.麻风树种子中毒蛋白的提取分离[J].化学研究与应用,2005,17(6):737-740.
作者姓名:梁渠  闫书一  李瑜  陈康生  何文琼  何丽丽
作者单位:成都理工大学材料与化学化工学院,四川,成都,610059
基金项目:四川省科技厅应用基础研究资助项目(02GY029011)
摘    要:研究了从麻风树种子中提取毒蛋白的过程。在冰水浴中用缓冲溶液在不同的pH值范围内对麻风树种子毒蛋白进行粗提,得到最高的提取率为98.2%。以改性聚丙烯酰胺与甲苯二异氰酸酯等进行混合发泡合成功能泡沫塑料。然后用合成的功能泡塑作为填料,用柱层析法对蛋白质进行分离,具有较好的分离效果。对分离得到的麻风树毒蛋白毒性进行检测,实验证明其有良好的杀虫杀菌效果。

关 键 词:麻风树  毒蛋白  功能泡沫塑料
文章编号:1004-1656(2005)06-0737-04
收稿时间:2004-12-03
修稿时间:2005-08-25

Study on extracting the poisonous protein from the seed of Jatropha
Qu liang,Shu yi-yan,Yu li,Kang sheng-chen,Wen qiong-he,Li li-he.Study on extracting the poisonous protein from the seed of Jatropha[J].Chemical Research and Application,2005,17(6):737-740.
Authors:Qu liang  Shu yi-yan  Yu li  Kang sheng-chen  Wen qiong-he  Li li-he
Institution:ChengDu University of Technology, Chengdu 610059,China
Abstract:The process of extracting the poisonous protein from the seed of Jatropha was studied.In order to avoid denaturation of the poisonous protein,the experiment was carried out under the condition of icewater and protection by thioethanol,with the different concentrations of cushioning solution and the various pH values.The highest extracting proportion was attained(98%).The functional polyether polyurethane foam was synthesized by mixing the polyacrylamide and toluene 2,4-diisocyanate,etc.The poisonous protein was separated from the mixed proteins by using the synthetic functional foam and the good separating result was achieved.At last the toxicity of poisonous protein was tested.The result indicated that it had good property of insecticide and sterilization.
Keywords:Jatropha  poisonous protein  the functional foam
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号