| Hg2+处理对经NBS修饰前后BSA光谱性质的影响 |
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| 引用本文： | 袁向华,孟乐,张年辉,杜林方.Hg2+处理对经NBS修饰前后BSA光谱性质的影响[J].化学研究与应用,2004,16(3):333-335. |
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| 作者姓名： | 袁向华 孟乐 张年辉 杜林方 |
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| 作者单位： | 四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064 |
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| 摘 要： | pH7.0时Hg^2 处理导致BSA紫外吸收增加，出现LMCT带，强度随处理时间增加而增强，蛋白质内源荧光强度下降。而Hg^2 处理对经NBS修饰的BSA的紫外吸收LMCT带强度随处理时间增加而降低，278nm波长光激发所得最大荧光发射峰位置出现蓝移，也与处理时间相关。表明重金属Hg^2 对BSA除Trp残基附近外还有多个作用部位。
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| 关 键 词： | Hg2+处理 牛血清白蛋白 BNS修饰 光谱性质 |
| 文章编号： | 1004-1656(2004)03-0333-03 |
| 修稿时间： | 2003年9月30日 |
Effect of Hg2+ on the spectra characterization of BSA with or withou NBS modification |
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| YUAN Xiang-hua,MENG Le,ZHANG Nian-hui,DU Lin-fang.Effect of Hg2+ on the spectra characterization of BSA with or withou NBS modification[J].Chemical Research and Application,2004,16(3):333-335. |
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| Authors: | YUAN Xiang-hua MENG Le ZHANG Nian-hui DU Lin-fang |
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| Institution: | YUAN Xiang-hua,MENG Le,ZHANG Nian-hui,DU Lin-fang~* |
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| Abstract: | In neutral condition(PH 7.0),Hg~(2+) treatment led to the increasing of UV absorption of BSA,and LMCT band was induced.The intensity of LMCT band was enhanced as the treating time course.But the change pattern of protein intrinsic fluorescence intensity was quite different.However,the treatment of Hg~(2+) on NBS-modified BSA had different effects on the UV absorption spectrum and protein intrinsic fluorescence spectrum:the intensity of LMCT band decreased along with the treating time and the emission fluorescence peak that excited at 278 nm got a blue shift which was also relative to the treating time.All these results indicated that heavy metal ion Hg~(2+) has multiple effecting sites on BSA. |
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| Keywords: | Hg~(2+) bovine serum albumin NBS modification Spectra characterization |
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