Synthesis,characterization and biodistribution of 99mTc-Bioquin-HMPAO (99mTc-BH) as a novel brain imaging agent |
| |
Authors: | Ayfer Yurt Kilcar Fazilet Zumrut Biber Muftuler Huseyin Enginar Volkan Tekin Emin Ilker Medine Perihan Unak |
| |
Institution: | 1. Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100, Bornova, Izmir, Turkey 2. Department of Chemistry, Afyon Kocatepe University, Afyon, Turkey
|
| |
Abstract: | Recently, the development of novel brain imaging agents has aroused much interest thanks to limited number of brain cancer or diseases diagnosis agents. It is aimed to synthesize a novel brain imaging agent including a promise for further studies on AD diagnosis potential and investigate its bioaffinity with biodistribution studies on healthy Balb/c mice. A novel radiolabeled agent was synthesized and characterized. Quality control of 99mTc-BH was performed utilizing solvent extraction and chromatographic (Radio TLC and Radio HPLC) methods. Bioaffinity of the 99mTc-BH was investigated on male Balb/c mice at various time points (5, 30, 60, 120 min post-injection). Paper electrophoresis showed that 99mTc-BH has a neutral structure. Radiochemical purity of 99mTc-BH was over 95 % with appropriate stability for imaging period. Selected brain regions have uptakes over 4 % ID/g following intravenous injection. Hippocampus has uptake approximately 10 % ID/g. 99mTc-BH has shown brain uptake, so it may prove to be valuable for brain imaging as a novel technetium-labeled agent. Further investigations with AD animal model are our on going effort to show that this agent has AD diagnosis potential. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|