Secondary-Ion Mass Spectrometry Images Cardiolipins and Phosphatidylethanolamines at the Subcellular Level |
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| Authors: | Dr Hua Tian Dr Louis J Sparvero Paul Blenkinsopp Dr Andrew A Amoscato Prof Simon C Watkins Prof Hülya Bay?r Prof Valerian E Kagan Prof Nicholas Winograd |
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| Institution: | 1. Department of Chemistry, Pennsylvania State University, 209 Chemistry Bldg., University Park, PA, 16802 USA;2. Department of Environmental and Occupational Health and Center for Free Radical and Antioxidant Health, University of Pittsburgh, USA;3. Ionoptika Ltd., Unit B6, Millbrook Cl, Chandler's Ford, Eastleigh, SO53 4BZ UK;4. Department of Cell Biology, University of Pittsburgh, USA |
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| Abstract: | Millions of diverse molecules constituting the lipidome act as important signals within cells. Of these, cardiolipin (CL) and phosphatidylethanolamine (PE) participate in apoptosis and ferroptosis, respectively. Their subcellular distribution is largely unknown. Imaging mass spectrometry is capable of deciphering the spatial distribution of multiple lipids at subcellular levels. Here we report the development of a unique 70 keV gas-cluster ion beam that consists of (CO2)n+(n>10 000) projectiles. Coupled with direct current beam buncher-time-of-flight secondary-ion mass spectrometry, it is optimized for sensitivity towards high-mass species (up to m/z 3000) at high spatial resolution (1 μm). In combination with immunohistochemistry, phospholipids, including PE and CL, have been assessed in subcellular compartments of mouse hippocampal neuronal cells and rat brain tissue. |
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| Keywords: | Cardiolipin Ferroptose Gascluster-Ionenstrahl Sekundärionen-Massenspektrometrie Subzelluläre Bildgebung |
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