| Institution: | 1. School of Chemistry and Chemical Engineering and Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601 China
These authors contributed equally to this work.;2. School of Chemistry and Chemical Engineering and Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601 China;3. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031 China;4. School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui, 243032 China;5. Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026 China;6. Department of Physics, Chemistry and Biology, Linköping University, Linköping, 58183 Sweden |
| Abstract: | Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H2O2 level leads to disassociation of nucleic acid–protein complexes. |
