Charge-Tagged DNA Radicals in the Gas Phase Characterized by UV/Vis Photodissociation Action Spectroscopy |
| |
| Authors: | Yue Liu Dr Andy Dang Dr Jan Urban Prof?Dr Franti?ek Ture?ek |
| |
| Institution: | 1. Department of Chemistry, University of Washington, Seattle, WA, 98195-1700 USA;2. Metagenics, Inc., Gig Harbor, WA, 98332 USA |
| |
| Abstract: | Adenosine radicals tagged with a fixed-charge group were generated in the gas phase and structurally characterized by tandem mass spectrometry, deuterium labeling, and UV/Vis action spectroscopy. Experimental results in combination with Born–Oppenheimer molecular dynamics, ab initio, and excited-state calculations led to unambiguous assignment of adenosine radicals as N-7 hydrogen atom adducts. The charge-tagged radicals were found to be electronically equivalent to natural DNA nucleoside radicals. |
| |
| Keywords: | Ladungsmarkierte Adenosinradikale Übergangszustände UV/Vis-Aktionsspektroskopie Vibronische Spektren Wasserstoffverlust-Kinetik |
|
|
