Fabrication of Pascal-triangle Lattice of Proteins by Inducing Ligand Strategy |
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| Authors: | Rongying Liu Dr Zdravko Kochovski Long Li Yue-wen Yin Jing Yang Guang Yang Guoqing Tao Anqiu Xu Ensong Zhang Prof Dr Hong-ming Ding Prof Dr Yan Lu Prof Dr Guosong Chen Prof Dr Ming Jiang |
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| Institution: | 1. The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University, Shanghai, 200433 China;2. Institute of Electrochemical Energy Storage, Helmholtz-Zentrum Berlin für Materialien und Energie, 14109 Berlin, Germany;3. Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, 215006 China |
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| Abstract: | A protein Pascal triangle has been constructed as new type of supramolecular architecture by using the inducing ligand strategy that we previously developed for protein assemblies. Although mathematical studies on this famous geometry have a long history, no work on such Pascal triangles fabricated from native proteins has been reported so far due to their structural complexity. In this work, by carefully tuning the specific interactions between the native protein building block WGA and the inducing ligand R-SL , a 2D Pascal-triangle lattice with three types of triangular voids has been assembled. Moreover, a 3D crystal structure was obtained based on the 2D Pascal triangles. The distinctive carbohydrate binding sites of WGA and the intralayer as well as interlayer dimerization of RhB was the key to facilitate nanofabrication in solution. This strategy may be applied to prepare and explore various sophisticated assemblies based on native proteins. |
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| Keywords: | inducing ligand pascal triangle self-assembly wheat germ agglutinin |
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