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The endocannabinoid anandamide is a precursor for the signaling lipid N-arachidonoyl glycine by two distinct pathways |
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| Authors: | Heather B Bradshaw Neta Rimmerman Sherry Shu-Jung Hu Valery M Benton Jordyn M Stuart Kim Masuda Benjamin F Cravatt David K O'Dell J Michael Walker |
| Institution: | (1) The Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA;(2) The Gill Center for Biomolecular Science, Indiana University, Bloomington, IN, USA;(3) The Kinsey Institute for Research in Sex, Gender and Reproduction, Indiana University, Bloomington, IN, USA;(4) The Scripps Institute, La Jolla, CA, USA |
| Abstract: | BackgroundN-arachidonoyl glycine (NAGly) is an endogenous signaling lipid with a wide variety of biological activity whose biosynthesis is poorly understood. Two primary biosynthetic pathways have been proposed. One suggests that NAGly is formed via an enzymatically regulated conjugation of arachidonic acid (AA) and glycine. The other suggests that NAGly is an oxidative metabolite of the endogenous cannabinoid, anandamide (AEA), through an alcohol dehydrogenase. Here using both in vitro and in vivo assays measuring metabolites with LC/MS/MS we test the hypothesis that both pathways are present in mammalian cells. |
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