环糊精修饰聚酰胺-胺树状高分子的合成及其与乳酸左氧氟沙星的作用研究

合成了一系列以环糊精修饰的树状高分子化合物PAMAM(G2,G4)-β-CD,用IR,1H-NMR等手段表征了其结构,并采用荧光光谱法对其在缓冲溶液中与乳酸左氧氟沙星(LFL)的相互作用进行了研究.结果表明,经环糊精修饰树状高分子的增敏率远大于未修饰的和天然环糊精,且随代数和环糊精含量的增加而增大,表明其具有强于相同代数PAMAM的分子键合能力,这些强的键合能力源于环糊精修饰树状高分子化合物中两种结构单元的疏水作用、静电作用和氢键作用的协同效应.

SYNTHESIS OF POLYAMIDOAMINE DENDRIMERS MODIFIED BYCYCLODEXTRIN AND THEIR MOLECULAR INCLUSION INTERACTION WITH LEVOFLOXACIN LACTATE

The polyamidoamine (PAMAM)dendrimers having different generations (G2 and G4)modified by β-cyclodextrin (β-CD),PAMAM( G2,G4)-β-CD,were synthesized using substitution reaction from mono-6- iodine-β-cyclodextrin and PAMAM dendrimers,and the molecular structures were characterized by 1H-NMR and IR.The molecular interaction between various dendrimers and levofloxacin lactate ( LFL) was determined by fluorimetry in buffer solution (pH = 7.4)at 25℃ .It was found that the PAMAM(G2,G4)-β-CD possesses higher sensitizing ability than that of the corresponding parent dendrimers and natural β-CD,and it increases concomitantly with the increases of generation and content of β-CD,suggesting that the PAMAM( G2,G4)-β- CD possesses strong inclusion ability with LFL.The possible interaction mechanism between PAMAM-CD and LFL was proposed by 1H-NMR analysis and theoretical calculation.The results showed that the LFL molecule was placed in the amino end of dendrimer molecule and between the sides of cyclodextrin cavities to form supramolecular complexes.Furthermore,the results indicate that the main driving force of the system could be attributed to the electrostatic interaction as well as hydrogen bonding between the LFL and PAMAM-CD,and fully reflects the synergistic effect of intermolecular forces.