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以光诱导Fenton反应控制M-PEIs纳米凝胶的粒径
引用本文:徐冬梅,洪军,孙汉文,宫培军,董黎,盛康龙,姚思德.以光诱导Fenton反应控制M-PEIs纳米凝胶的粒径[J].高分子学报,2007,0(4):389-393.
作者姓名:徐冬梅  洪军  孙汉文  宫培军  董黎  盛康龙  姚思德
作者单位:1. 中国科学院上海应用物理研究所,上海,201800;中国科学院研究生院,北京,100039
2. 中国科学院上海应用物理研究所,上海,201800
基金项目:上海市纳米科技专项基金 , 中国科学院前沿领域研究基金
摘    要:基因治疗,就是将正常基因导入体内以弥补缺失或替换突变基因来医治囊性纤维化和血友病等1]多种疾病.而制备高效安全的基因转染载体至今仍是基因治疗的一个瓶颈.非病毒类载体的基因转染效率目前虽不高,但由于其具有低毒、低免疫反应、无基因插入片断大小限制、制备方便、易保存

关 键 词:光化学  聚乙烯亚胺  非病毒载体  紫外线  光助Fenton反应
收稿时间:2006-04-17
修稿时间:2006-08-15

SIZE CONTROLLING OF M-PEIS NANOGELS via PHOTO-INDUCED FENTON REACTION
XU Dongmei,HONG Jun,SUN Hanwen,GONG Peijun,DONG Li,SHENG Kanglong,YAO Side.SIZE CONTROLLING OF M-PEIS NANOGELS via PHOTO-INDUCED FENTON REACTION[J].Acta Polymerica Sinica,2007,0(4):389-393.
Authors:XU Dongmei  HONG Jun  SUN Hanwen  GONG Peijun  DONG Li  SHENG Kanglong  YAO Side
Institution:1 Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800;2 Graduate School of Chinese Academy of Sciences, Beijing 100039
Abstract:Photo-induced Fenton reaction was utilized in this paper to control the sizes of polyethylenimine hydrogels (M-PEIs,M,Fe atom) as gene delivery vector.H_2O_2 and FeCl_2 were mixed with PEI pre_polymer in water in a 3-neck quartz flask, and the mixture was stirred vigorously.M-PEIs nanogels dispersed very well in water could be prepared under illumination by use of low-pressure Hg lamps at room temperature.This system was protected from oxygen with N_2.By changing reaction conditions,such as the pH value,reaction time and concentrations of solutes,sizes of M-PEIs nanogels could be controlled.It was found that the sizes of M-PEIs decreased with the pH value and the concentrations of FeCl_2.The increasing of the initiator (H_2O_2) concentrations would make the particles more tighten,but might result in crosslinking or degradation of particles.A suitable irradiation time ensured formation of homogeneous nanogel products,but prolonged irradiation would cause degradation of nanogels.After photo crosslinking,the products were purified with membrane filters of 0.45 μm and treated further by dialysis bags with size exclusion below 10 kDa in order to make them more stable for storage.
Keywords:Photochemistry  Polyethyleneimine  Nonviral vector  UV  Photo-Fenton reaction
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