大核树枝状大分子的合成及其凝血、溶血性能研究

从 8 0年代中期开始 ,Ｔｏｍａｌｉａ、Ｎｅｗｋｏｍｅ、Ｆｒｅｃｈｅｔ等对树枝状大分子开展了卓有成效的研究 ,合成了多种结构的树枝状大分子1 ] ,并对它们的应用进行了积极的探讨 .在数枝状大分子的合成方面 ,寻找新的多官能团引发核就是一个研究热点 ,例如采用大分子核 ,Ｆｒｅｃｈｅｔ等采用聚乙二醇作为核用收敛法合成了聚芳醚和聚芳酯树枝状大分子2 ,3] ,为嵌段共聚增添了新的内容 .而Ｔｏｍａｌｉａ等以氨和乙二胺等小分子为核合成的聚酰胺 胺类树枝状大分子呈粘糊状4] ,取样、称量等操作很麻烦 ,其应用也受到限制 .聚乙二醇无毒…

SYNTHESIS OF DENDRIMERS WITH MACRO CORE AND RESEARCH ON THEIR PROPERTIES OF COAGULATION AND HEMOLYSIS

In order to obtain the inviscid dendrimers with more terminal amino-groups than those of ω,ω′-polyethylene glycol diamine, in the meanwhile to untilize the fine biocompatibility of polyethylene glycol,a series of polyamidoamine dendrimers with polyethylene glycol core were synthesized by Tomalia's divergent method which invovled a repeated two-step process:(1) exhaustive Micheal addition of ω,ω′-polyethylene glycol diamine as the initiating core,with methyl acrylate and (2) exhaustive amidation of the resulting esters with a large excess of ethylenediamine. The structures of all generations of the dendrimer was identified by FT-IR and elemental analysis, and the numbers of the terminal aminogroups of the integral generations of the dendrimer were determined by potentiometer titration.Although PEG-NH-2 and the integral generations had adsorption at ～3300 cm-1 in FT-IR, the half generations did not have. It indicated that all the steps of Micheal addition took place thoroughly.On the other hand, the conversion of the half generations into their corresponding integral generations made the adsorption of the ester's carbonyl at 1735～1740cm-1 disappear,it suggested that the conversion was complete.The results of elemental analysis also affirmed the above discussion.However the data of elemental analysis and potentiometer titration of PEG-5^0 and PEG-6^0 didn't entirely coincide with their theoretical values,that may be due to the residue of the ingredients such as ethylenediamine which were bundled up by larger generations with more branches and difficult to remove.Except that PEG-1^0 and PEG-2^0 are solids,the other integral generations are viscous. Influences of the integral generations on coagulation and hemolysis were examined,and the results showed that the properties of coagulation and hemolysis of the integral generations of the dendrimer were similar to those of polyehylene glycol.