用水溶性四苯基乙烯基荧光探针检测ctDNA

设计合成了一种水溶性的四苯基乙烯(TPE)衍生物TPEDPyMe,研究了该化合物的吸收和发射特性,发现TPEDPyMe具有聚集诱导发光(AIE)性能.在pH值为7.2的三羟甲基氨基甲烷-盐酸(tris-HCl)缓冲溶液中用TPEDPyMe检测小牛胸腺DNA(ctDNA)时观察到,随着ctDNA的浓度从0μg/mL增大到...

DETECTION OF ctDNA WITH WATER SOLUBLE TETRAPHENYLENE-BASED FLUORESCENCE PROBE

A water soluble cationic fluorescent probe(TPEDPyMe) was designed and synthesized by attaching two pyridinium moieties onto the tetraphenylethene(TPE) core.The molecular structure of TPEDPyMe was characterized by multiple spectroscopic techniques,and its fluorescence property was examined.As its maternal compound,TPEDPyMe exhibited typical aggregation-induced emission(AIE) behavior,and the emission peaked at around 630 nm in aqueous solution.TPEDPyMe was used as a fluorescent probe to detect ctDNA in tris-HCl buffer solution at a pH value of 7.2.It was found that the fluorescence intensity of the TPEDPyMe in the buffer solution was boosted up to 20 folds when the concentration of ctDNA increased from 0 μg/mL to 500 μg/mL.Meanwhile,the emission peaks blue-shifted from 627 nm to 576 nm.In the concentration region from 0 μg/mL to 50 μg/mL,the change in fluorescence intensity was linearly proportional to the concentration of ctDNA.In this region,the Stern-Volmer constant or KSV was calculated to be 1.4 × 102,showing a desirable sensitivity of TPEDPyMe to ctDNA.Moreover,when the concentration of ctDNA increased from 0 μg/mL to 500 μg/mL,the emission peak shifted from 633 nm to 578 nm.This phenomenon implies that the TPEDPyMe can be used as a dual-responsive fluorescent label for probing ctDNA,which is of great significance in biosensor technologies.In summary,the present results indicate that TPEDPyMe is a promising fluorescent probe for the detection of DNA species and more detailed investigation is going on in the laboratory.